二至丸抗大鼠肾细胞衰老的GC-MS代谢组学研究

目的?研究D-半乳糖诱导的衰老大鼠肾细胞（NRK）内源性代谢物的变化，寻找与衰老有关的代谢生物标志物，探讨二至丸对衰老模型的调节作用及其抗衰老的可能机制。方法?采用D-半乳糖复制NRK细胞衰老模型，借助气相色谱-质谱联用（GC-MS）技术，对各组细胞样本进行测定，采用Kruskal-Wallis非参数检验及Dunn's多重比较进行单变量统计分析来筛选差异性代谢物。结果?经PLS-DA模式识别分析，对照组、模型组和EZW组细胞样本能够得到很好的区分，共鉴定出31种潜在生物标志物，二至丸含药血清给药后衰老NRK细胞胞内的内源性代谢物水平发生不同程度的回调。结论?二至丸可以使D-半乳糖诱导的衰老NRK细胞胞内的异常代谢有所恢复，其治疗作用可能与机体内31个代谢物及10条相关代谢通路的调节有关。 

Metabolomics Study of Erzhiwan Against Normal Rat Kidney Cell Senescence Based on GC-MS

OBJECTIVE?To investigate the changes of endogenous metabolites in senile rat kidney cells (NRK) induced by D-galactose， to find metabolic biomarkers related to aging and to explore the regulation of Erzhiwan on aging model and the anti-aging effect of Erzhiwan. METHODS?The senescence model was constructed by D-galactose (D-gal). The gas chromatography-mass spectrometry (GC-MS) technique was used to determine the cell samples in each group. Univariate statistical analysis was performed using the Kruskal-Wallis nonparametric test and Dunn's multiple comparison. RESULTS?PLS-DA pattern recognition analysis showed that the cell samples of the control group， the model group and the EZW group could be well distinguished. A total of 31 potential biomarkers were identified， and the endogenous metabolite levels of the senescent NRK cells were varying degrees of callbacks after the administration of the drug-containing serum. CONCLUSION?Erzhiwan could restore the abnormal metabolism of D-galactose-induced senescent NRK cells， and its therapeutic effect may be related to the regulation of 31 metabolites and 10 related metabolic pathways.