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Alopecurone B逆转人乳腺癌细胞MCF-7阿霉素耐药株活性研究
引用本文:黄莉莉,王欣,李冰冰,缪明星. Alopecurone B逆转人乳腺癌细胞MCF-7阿霉素耐药株活性研究[J]. 南京中医药大学学报, 2019, 35(6): 699-703
作者姓名:黄莉莉  王欣  李冰冰  缪明星
作者单位:1.南京大学医学院附属鼓楼医院,江苏 南京 210008
摘    要:目的 研究黄酮化合物Alopecurone B(APB)对人乳腺癌细胞阿霉素耐药株MCF-7/ADR多药耐药的逆转活性及逆转机制。方法 人乳腺癌细胞阿霉素耐药株MCF-7/ADR维持在含有250 ng/mL阿霉素的培养基中,使用MTT法、qPCR、Western blot和流式细胞术研究APB对耐药株P-糖蛋白(P-gp)功能和表达的影响。结果 APB能逆转MCF-7/ADR细胞多药耐药,抑制P-gp的功能,下调P-gp基因和蛋白的表达。结论 APB具有强效逆转人乳腺癌细胞MCF-7/ADR多药耐药的效果,其机制涉及对P-gp的调控。 

关 键 词:Alopecurone B   阿霉素   乳腺癌   多药耐药   P-糖蛋白

Reversal Effect and Mechanism of Alopecurone B on MCF-7 Adriamycin-Resistant Subline
HUANG Li-li,WANG Xin,LI Bing-bing,MIAO Ming-xing. Reversal Effect and Mechanism of Alopecurone B on MCF-7 Adriamycin-Resistant Subline[J]. Journal of Nanjing University of Traditional Chinese Medicine(Natural Science), 2019, 35(6): 699-703
Authors:HUANG Li-li  WANG Xin  LI Bing-bing  MIAO Ming-xing
Affiliation:1.Drum Tower Hospital Affiliated to School of Medicine, Nanjing University, Nanjing, 210008, China2.School of pharmacy, China Pharmaceutical University, Nanjing, 210009, China
Abstract:OBJECTIVE To study the reversal effect and the mechanism of APB on adriamycin (ADR)-induced multidrug-resistant human breast cancer MCF-7/ADR cells. METHODS ADR-resistant MCF-7 cells were maintained in RPMI-1640 culture medium containing 10%FBS and 250 ng/mL ADR. MTT assay, qPCR, Western blot and flow cytometry were used to determine the inhibitory effect of APB on P-gp levels and function in MCF-7 or MCF-7/ADR cells. RESULTS APB reversed MCF-7/ADR multidrug resistance at the concentration of 10 μmol/L. Treated with 0, 5, 10, 20 μmol/L of ABP, P-gp gene and protein levels were suppressed significantly in a concentration-dependent manner. Furthermore, APB in 10 μmol/L markedly inhibited the function of P-gp. CONCLUSION APB potent reversed the multidrug resistance in MCF-7 ADR-resistant subline. The mechanism referred to P-gp inhibition. 
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