葡萄内脂对PC12神经细胞损伤的保护作用研究

目的探讨葡萄内脂对过氧化氢(H2O2)诱导的肾上腺嗜铬瘤细胞(PC12)神经细胞损伤的保护作用。方法体外培养PC12细胞,建立PC12细胞过氧化氢损伤模型。将处于对数生长期的PC12细胞分为正常对照组、模型组、阳性对照组、5μmol/L葡萄内脂组、25μmol/L葡萄内脂组、50μmol/L葡萄内脂组。采用不同浓度(5μm/L,25μm/L和50μm/L)葡萄内脂对PC12细胞进行干预,通过测定细胞存活率、细胞上清液中乳酸脱氢酶水平和细胞内氧化活性物质(ROS)水平评价干预效果。结果模型组PC12细胞存活率显著低于正常对照组及阳性对照组; 25μmol/L、和50μmol/L葡萄内脂组PC12细胞存活率(与阳性对照组相当)显著高于模型组,随着葡萄内脂浓度的增加,H2O2诱导损伤的PC12细胞的存活率呈升高趋势,组间比较差异有统计学意义(P <0. 05)。模型组PC12细胞上清液乳酸脱氢酶水平明显高于正常组及阳性对照组,5μmol/L、25μmol/L和50μmol/L葡萄内脂组细胞上清液乳酸脱氢酶水平(与阳性对照组相当)均显著低于模型组,且随着葡萄内脂浓度的增加,乳酸脱氢酶水平呈降低趋势,组间比较差异有统计学意义(P <0. 05)。模型组PC12细胞ROS水平显著高于正常对照组及阳性对照组,5μmol/L、25μmol/L和50μmol/L葡萄内脂组PC12细胞ROS水平(与阳性对照组相当)均显著低于模型组,且随着葡萄内脂浓度的增加,ROS水平呈降低趋势,差异有统计学意义(P <0. 05)。结论葡萄内脂对H2O2诱导损伤的PC12神经细胞具有明显的保护作用,可能与其能维持细胞膜的完整性、发挥抗氧化作用有关。

Study of protective effect of auraptene on PC12 nerve cells injury

Objective To explore the protective effect of auraptene on hydrogen peroxide(H2O2)-induced injury of PC12 nerve cells.Methods Rat adrenal PC12 cells were cultured in vitro,and a hydrogen peroxide(H2O2)-induced injury model of PC12 cells was established.The PC12 cells in the logarithmic growth phase were divided into normal control group,model group,positive control group,5μmol/L auraptene group,25μmol/L auraptene group,and 50μmol/L auraptene group.PC12 cells were treated with different concentrations(5μm/L,25μm/L and 50μm/L)of auraptene.The cell viability,lactate dehydrogenase levels in cell supernatants,and intracellular oxidative active substances(ROS)were determined to evaluate the protective effect of auraptene.Results The survival rate of PC12 cells in the model group was significantly lower than that in the normal control group and the positive control group.The survival rates of PC12 cells in the 25μmol/L auraptene group and 50μmol/L auraptene group(equivalent to the positive control group)were significantly higher than that in the model group,the survival rate of H2O2-induced injury PC12 cells increased with the raise of auraptene concentration,and the difference was statistically significant(P<0.05).The level of lactate dehydrogenase in PC12 cells supernatant in the model group was significantly higher than that in the normal group or the positive control group,and the levels of lactate dehydrogenase in PC12 cells supernatant of the 5μmol/L auraptene group,25μmol/L auraptene group and 50μmol/L auraptene group(equivalent to the positive control group)were significantly lower than that in the model group,the level of lactate dehydrogenase decreased with the raise of auraptene concentration,and the difference was statistically significant(P<0.05).The ROS level of PC12 cells in the model group was significantly higher than that in the normal control group or the positive control group,the ROS levels of PC12 cells in the 5μmol/L auraptene group,25μmol/L auraptene group and 50μmol/L auraptene groups(equivalent to the positive control group)were significantly lower than that in the model group,the ROS level decreased with the raise of auraptene concentration,and the difference was statistically significant(P<0.05).Conclusion Auraptene has obvious protective effect against H2O2-induced injury in PC12 nerve cells,which may be related to maintenance of cell membrane integrity and antioxidant activity.