基于动物温度趋向行为学的吴茱萸多组分寒热药性差异研究

目的 阐述热性中药吴茱萸中各组分的寒热药性差异。方法 选用KM小鼠，分别灌胃给予吴茱萸水提液及其不同洗脱部位，考察不同组分对小鼠温度趋向性的影响，并结合小鼠的肝脏ATP酶活力和能量代谢变化，探讨吴茱萸不同组分寒热药性。结果 与空白组比，60%醇洗部位、80%醇洗部位和吴茱萸水提液组小鼠在低温区的停留比例明显增高(P<0.05)；水洗组小鼠在高温区的停留比例明显增高(P<0.05)，表现出明显的趋热性；水洗组小鼠肝组织钠钾ATPase、镁ATPase活力均显著降低(P<0.05)；20%醇洗部位小鼠肝组织钙ATPase活力均显著提高(P<0.05)；60%醇洗部位、80%醇洗部位小鼠肝组织钠钾ATPase活力比空白组高，且有显著差异(P<0.05)；吴茱萸水提液组钠钾ATPase，钙ATPase较空白组高，且有显著差异(P<0.05)。与空白组比较，吴茱萸水提物组及其20%醇洗部位、60%醇洗部位和80%醇洗部位均增加了小鼠的耗氧量、产热量和能量代谢(P<0.05)；吴茱萸水洗脱部位降低了小鼠的耗氧量、产热量和能量代谢(P<0.05)。结论 吴茱萸所表现出的热性，是在寒性组分和热性组分相互作用后药性作用的综合或加和，其中包含了热性组分的热性抵抗寒性组分的寒性过程而最终体现出热性。 

Study on the Cold-Heat Nature of Multicomponents in Fructus Evodiae Based on the Ethology of Animal Temperature Tropism

OBJECTIVE To expound the cold and heat properties of different components in Evodia rutaecarpa. METHODS KM mice were orally administrated with Evodia rutaecarpa water extract or its different elution parts. The different elution parts on the temperature tropism of mice， the ATP enzyme activity in mice liver， and the energy metabolism in mice were carried out to evaluate their cold-heat nature. RESULTS Compared with the blank control group， the proportion of mice staying in the low temperature area was significantly increased under the treatment of 60% ethanol washed part， 80% ethanol washed part and Evodia rutaecarpa aqueous extract group (P<0.05). The retention rate of mice in the high temperature area was significantly higher in the washing group (P<0.05). The activities of Na-K-ATPase and Mg-ATPase in mice liver were significantly decreased (P<0.05) under the treatment of aqueous washing group. The activities of Ca-ATPase in mice liver of 20% group were significantly increased (P<0.05). The activity of Na-K-ATPase in mice liver of 60% and 80% group was higher than control group (P<0.05). The activity of Na-K-ATPase and Ca-ATPase of the Evodiae water extract group was higher than control group (P<0.05). Compared with the blank group， Evodia rutaecarpa water extract group and its 20%， 60% and 80% groups increased the mice oxygen consumption， heat production and energy metabolism (P<0.05). Evodia rutaecarpa water elution site reduced the consumption Oxygen， caloric and energy metabolism (P<0.05). CONCLUSION The thermal properties of Evodia rutaecarpa are the synthesis or addition of its cold and thermal components. Meanwhile， the thermal components resisted the cold nature of cold components and finally showed the nature of heat.