Enzymatic synthesis of [1‐14C‐N‐acetyl,P18O2] cytidine monophosphate neuraminic acid

A method for the enzymatic synthesis of 1‐¹⁴C‐N‐acetyl, P¹⁸O₂] cytidine monophosphate neuraminic acid (CMP‐NeuAc) is described. Central to the synthesis of 1‐¹⁴C‐N‐acetyl, P¹⁸O₂]CMP‐NeuAc was the enzymatic preparation of γ‐P¹⁸O₃]ATP for use in a reaction with uridine kinase and cytidine to provide 5′‐P¹⁸O₃]CMP. The 1‐¹⁴C‐N‐acetyl, P¹⁸O₂]CMP‐NeuAc isotopomer was then synthesized from a reaction involving nucleoside monophosphate kinase, pyruvate kinase and CMP‐NeuAc synthetase. The isolated reaction yield was 35%. Copyright © 2004 John Wiley & Sons, Ltd.