基于HMGB1探讨“易层”贴敷对KOA大鼠滑膜纤维化的影响

目的 基于HMGB1研究“易层”贴敷对膝骨关节炎（KOA）大鼠滑膜纤维化的影响。方法 30只SPF级SD大鼠，随机均分为空白对照组、模型组及易层组。KOA模型通过碘乙酸钠构建，造模成功后，易层组外敷“易层”贴敷28 d。末次给药后腹主动脉采血并提取各组滑膜组织，HE染色观察滑膜炎症；ELISA检测血清中促炎因子IL-1β、IL-6、TNF-α，抑炎因子IL-4、IL-10、TGF-β含量；Western blot和qPCR检测滑膜HMGB1及α-SMA、TIMP1、CollagenⅠ、CollagenⅢ蛋白和基因表达量。结果 HE染色显示易层组炎性细胞浸润较模型组显著减少，排列较为规则；模型组较空白对照组IL-1β、IL-6、TNF-α含量明显升高（P<0.05），易层组较模型组IL-1β、TNF-α含量明显降低（P<0.05），模型组较空白对照组IL-4含量明显升高（P<0.05），IL-10、TGF-β含量明显降低（P<0.05），易层组较模型组IL-10含量明显升高（P<0.05）；HMGB1及α-SMA、TIMP1、CollagenⅠ、CollagenⅢ的蛋白和基因水平在模型组较空白对照组表达增加（P<0.05），易层组较模型组表达下调（P<0.05）。结论 “易层”贴敷能够抑制滑膜纤维化，其作用机制可能与抑制HMGB1、平衡促炎因子和抑炎因子有关。 

Effect of Yiceng on the Synovial Fibrosis in KOA Rats Based on HMGB1

OBJECTIVE To investigate the effect of Yiceng on the synovial fibrosis in KOA rats based on HMGB1. METHODS SPFSD 30 rats were randomly devided into normal group， model group and Yiceng group equally. The KOA model by injected sodium iodoacetate into The knee. After modeling，The Yiceng group was applied Yiceng for 28 days. After the final administration， blood was collected from the abdominal aorta and synovial tissues of each group wereextracted， Synovitis was observed by HE staining; Detecting proinflammatory cytokines: IL-1β， IL- 6， TNF-α and Inflammatory cytokines: IL-4， IL-10， TGF-β by ELISA; then detected HMGB1， α-SMA， TIMP1， CollagenⅠand Collagen Ⅲ protein and gene expression in synovium by Western Blot and qPCR. RESULTS HE staining showed that inflammatory cell infiltration in the yiceng group was significantly reduced compared with the model group， and the arrangement was relatively regular.The contents of IL-1β，IL-6 and TNF-α in the model group were significantly higher than those in the blank control group (P<0.05)， and the contents of IL-1β， TNF-α in the yiceng group were significantly lower than those in the model group (P<0.05). The contents of IL-4 in the model group were significantly higher than those in the blank control group (P<0.05)， and the contents of IL-10 and TGF-β in the model group were significantly lower than those in the blank control group (P<0.05)， The contents of IL-10 in the yiceng group were significantly higher than those in the model group (P<0.05).the content of HMGB1，α-SMA，TIMP1，Collagen Ⅰ and Collagen Ⅲ protein and genelevel expressed in the model group were significantly higher than the blank group （P<0.05）; The content of HMGB1、α-SMA、TIMP1、Collagen Ⅰ and Collagen Ⅲ protein and gene level expression in the yiceng group were significantly lower than the model group（P<0.05）. CONCLUSION Yiceng can inhibit synovial fibrosis may be related to the inhibition of HMGB1 and the balance of pro-inflammatory and anti-inflammatory factors.