糖痹康对糖尿病大鼠细胞因子表达的影响

目的 观察糖痹康对糖尿病大鼠肿瘤坏死因子（TNF-α）、单核细胞趋化蛋白（MCP-1）、血管细胞粘附分子1（VCAM-1）、血氧化低密度脂蛋白（oxLDL）及细胞间粘附分子1（ICAM-1）表达影响。方法 采用高脂饲料喂养SD大鼠，注射STZ诱发2型糖尿病模型，给予糖痹康干预16周后，采集腹主动脉血和大鼠一侧坐骨神经样本，采用ELISA法检测血清细胞因子含量，Western blot法检测坐骨神经组织中MCP-1、VCAM-1及TNF-α表达。结果 与正常组相比，模型组大鼠坐骨神经组织TNF-α、VCAM-1和MCP-1蛋白表达以及血清中oxLDL、ICAM-1、VCAM-1、TNF-α和MCP-1含量均显著升高(P<0.01)；与模型组相比，糖痹康可显著降低组织和血清中细胞因子表达(P<0.05～0.01)，且呈现剂量依赖性。结论 糖痹康可下调STZ诱导的糖尿病大鼠血清TNF-α、oxLDL、ICAM-1、VCAM-1及MCP-1的含量和坐骨神经中VCAM-1、TNF-α、MCP-1蛋白表达，这可能是糖痹康对糖尿病周围神经病变神经保护的机制之一。 

Effect of Tangbikang on Cytokines in Diabetic Rats

OBJECTIVE To observe the traditional Chinese medicine compound Tangbikang in diabetic rats tumor necrosis factor (TNF-α)， monocyte chemotactic protein (MCP-1)， vascular cell adhesion molecule 1 (VCAM 1)， blood oxidized low density lipoprotein (oxLDL) and intercellular adhesion molecule 1 (ICAM - l) expression. METHODS SD rats were fed with high-fat diet， injected with STZ to induce type 2 diabetes， and 16 weeks after intervention with of Tangbikang， abdominal aortic blood and samples of one side of the sciatic nerve of the rats were collected， serum cytokine levels were detected by ELISE method， and expressions of MCP-1，VCAM-1 and TNF-α in sciatic nerve tissues were detected by Western blot. RESULTS Compared with the normal group， the expression of TNF-α， VCAM-1 and MCP-1 in the sciatic nerve tissues of the model group and the content of oxLDL， ICAM-1， VCAM-1， TNF-α and MCP-1 in the serum of the model group were significantly increased (P<0.01). Compared with the model group， Tangbikang significantly reduced the expression of cytokines in tissues and serum (P<0.05，P<0.01) in a dose-dependent way. CONCLUSION Tangbikang can down-regulate the contents of serum TNF-α， oxLDL， ICAM-1，VCAM-1 and MCP-1 in STZ-induced diabetic rats and the expression of VCAM-1， TNF-α and MCP-1 in the sciatic nerve， which may be one of the mechanisms of Tangbikang's neuroprotection in diabetic peripheral neuropathy.