| 基因扩增技术检测急慢性B—淋巴细胞白血病免疫球蛋白基因重排 |
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| 引用本文： | 董作仁,李栋梁,徐世荣,潘凌,张瑞欣,李贵彬,姜玲玲,温进坤,魏素珍,姚尔固. 基因扩增技术检测急慢性B—淋巴细胞白血病免疫球蛋白基因重排[J]. 白血病．淋巴瘤, 1993, 0(1) |
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| 作者姓名： | 董作仁 李栋梁 徐世荣 潘凌 张瑞欣 李贵彬 姜玲玲 温进坤 魏素珍 姚尔固 |
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| 作者单位： | 河北医学院二院，河北医学院二院，河北医学院二院，河北医学院二院，河北医学院二院，河北医学院二院，河北医学院基础所，河北医学院基础所，河北医学院基础所，河北医学院基础所 050000，050000，050000，050000，050000，050000 |
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| 摘 要： | 应用聚合酶链反应(PCR)扩增IgH重排产生的CDR—Ⅲ序列,检测了23例急慢性B—淋巴细胞白血病患者,20例发生IgH重排,其中4例出现两种基因扩增产物。6例完全缓解的患者,3例检出异常重排的IgH基因,并先后复发。本文还用Southern blot法与PCR进行了对照性研究。
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| 关 键 词： | 白血病 免疫球蛋白基因重排 聚合酶链反应 微小残留病 |
DETECTION OF GENE REARRANGEMENT OF IMMUNOGLOBULIN IN ACUTE AND CHRONIC LYMPHOID LEU KEMIAS OF B-CELL LINEAGE |
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| Dong Zuoren,et al The Second Affiliated Hospital of Hebei Medical College. DETECTION OF GENE REARRANGEMENT OF IMMUNOGLOBULIN IN ACUTE AND CHRONIC LYMPHOID LEU KEMIAS OF B-CELL LINEAGE[J]. Journal of Leukemia & Lymphoma, 1993, 0(1) |
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| Authors: | Dong Zuoren et al The Second Affiliated Hospital of Hebei Medical College |
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| Abstract: | Polymerase chain reaction (PCR) was used to amplify the complementarity determiningregion 3 (CDR--3) of immunoglobulin gene heavy (IgH). Among 23 cases of ALL and CLLpatients detected, IgH rearrangment occured in 20 cases, and 2 kinds of gene amplified prod-ucts appeared in 4 of them. Among 6 complete remission patients, abnormal rearranged IgHgene was detected in 3 of them successively. We had also done control research betweensouthern blot method and PCR technique. |
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| Keywords: | leukemia Polymerase chain reaction gene rearrangement of immunogulin minimal residual leukemia |
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