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| 一种念珠菌蛋白质的提取方法 | |
| 引用本文： | 焦立新,张云峰,贺丹,张波,宋文刚,王丽.一种念珠菌蛋白质的提取方法[J].中国组织工程研究与临床康复,2007,11(8):1540-1541. |
| 作者姓名： | 焦立新 张云峰 贺丹 张波 宋文刚 王丽 |
| 作者单位： | 1. 吉林大学基础医学院病原生物学教研室,吉林省长春市,130021;长春市中心血站,吉林省长春市,130041 2. 吉林大学基础医学院病原生物学教研室,吉林省长春市,130021;吉林大学第一医院,吉林省长春市,130021 3. 吉林大学基础医学院病原生物学教研室,吉林省长春市,130021 4. 吉林大学基础医学院病原生物学教研室,吉林省长春市,130021;北华大学医学院,吉林省吉林市,132000 |
| 摘 要： | 对蛋白质的提取方法进行优化,以便进行念珠菌蛋白质组研究。为使细胞壁有效破碎,除用玻璃珠涡旋、振荡外,加用玻璃棒研磨,提取的蛋白质经过以TCA/丙酮为基础的沉淀、纯化后进行双向电泳。用此方法提取热带、季也蒙、光滑3种念珠菌获得的蛋白质浓度基本相同;玻璃棒研磨能够提高蛋白质的浓度;蛋白质纯化可去除蛋白溶液中的杂质,双向电泳图谱效果良好,没有划线和拖尾。该方法适用于念珠菌蛋白质提取,所获得的蛋白质能够满足双向电泳分析的要求。 |
| 关 键 词： | 念珠菌 白色 念珠菌 光滑 念珠菌 热带 电泳 凝胶 双向 蛋白质组 |
| 修稿时间： | 2006-08-17 |
A method to extract protein of Candida |
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| Jiao Li-xin,Zhang Yun-feng,He Dan,Zhang Bo,Song Wen-gang,Wang Li.A method to extract protein of Candida[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2007,11(8):1540-1541. | |
| Authors: | Jiao Li-xin Zhang Yun-feng He Dan Zhang Bo Song Wen-gang Wang Li |
| Institution: | 1Department of Pathogenobiology, Basic Medical College, Jilin University, Changchun 130021, Jilin Province, China; 2Changchun Central Blood Station, Changchun 130041, Jilin Province, China; 3First Hospital of Jilin University, Changchun 130021, Jilin Province, China; 4Beihua University Medical College, Jilin 132000, JiUn Province, China |
| Abstract: | The method of protein extraction is optimized to study Candida protein group, Besides glass bead was used to vortex and vibrate, glass rod grind was applied to grind to break cell wall into pieces more effectively. After precipitated and purified with TCA/acetone, the extracted protein was subjected to two-dimensional gel electrophorasis. With this extraction method, the protein concentration involving Monilia guilliermondi. Candida tropicals and Candida glabrata was roughly the same, Glass rod grinding can improve the concentration of protein; protein purification can exclude the foreign material from protein solution and the results of two-dimensional electrophoregram are fine without streaking or tailing, This method is suitable for the protein extraction of Candida, and the extracted protein can meet the requirement of two-dimensional electrophoresis analysis. |
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