知母中芒果苷提取方法考察及知母毛皮肉中芒果苷含量比较

目的考察知母中芒果苷的提取方法并比较知母毛皮肉中芒果苷的含量。方法采用HPLC法比较不同溶剂、不同提取方法从知母中提取出的芒果苷的含量及知母毛皮肉中芒果苷含量。结果采用50%乙醇、超声提取的方法提取较完全且简便易行,毛皮肉中芒果苷的含量分别为0.305%、0.519%、0.826%。结论本方法中芒果苷与其它成分能很好地分离开,且快速、准确、重现性好。知母毛皮肉中均含一定量的芒果苷。

Study on the Extracting Method of Mangiferin in Anemarrhena Asphodeloides Bge and Comparison of Content of Mangiferin in Hair, Skin and Meat of Anemarrhena Asphodeloides Bge

Objective To study the extracting method of mangiferin in Anemarrhena asphodeloides Bge and compare the content of mangiferin in hair,skin and meat of Anemarrhena asphodeloides Bge. Methods Use HPLC to compare the content of mangiferin exacted from different solvents and by different extracting method and then compare the content of mangiferin in hair skin and meat of Anemarrhena asphodeloides Bge. Results The method using 50% alcohol and ultrasound can entirely extract and the content of mangiferin in hair skin and meat of Anemarrhena asphodeloides Bge is 0.298%,0.538% and 0.826% respectively. Conclusion Using this method to determine content of Mangiferin in Anemarrhena asphodeloides Bge is simple,rapid, accurate and can be repeated. There is certain content of mangiferin in hair, skin and meat of Anemarrhena asphodeloides Bge.