~(131)Ⅰ标记抗卵巢上皮癌单克隆抗体进行人卵巢癌裸鼠移植瘤放射免疫显像

用~(131)Ⅰ标记自行制备的抗卵巢上皮癌单克隆抗体COC166-9及COC183-B_2,并对人卵巢癌裸鼠移植瘤进行放射免疫显像,还观察裸鼠体内的生物学分布,注射标记单克隆抗体后48～72小时放射免疫显像呈阳性图象。其生物学分布:T/B为1.09,T/NT为4.38;而对照组T/B为0.16,T/NT为1.1。说明这种单抗对卵巢上皮癌有亲和性。

RADIOIMAGING AND BIOLOGICAL DISTRIBUTION OF HUMAN OVARIAN CARCINOMA XENOGRAFT IN NUDE MICE WITH ~(131)I LABELED MONOCLONAL ANTIBODIES

131I-labeled anti-ovarian epithelial adenocarcinoma monoclonal antibodies (McAb) COC166-9, COC183 BI raised in our lab were used to investigate the localization and biological distribution in human ovarian carcinoma xenograft in nude mice.Radioimaged tumor had been shown 24 hours after administration of 131I-labeled McAb. It was more clear 48 to 72 hours later. The localizations and the sizes of the tumor could be clearly visualized in the gamma scintigrams which were corresponding with the tumors grown in the nude mice. Negative results were found in control groups. Tumor/blood ratios were determined at 24, 48, 72 and 96 hours respectively after injections of 131I-COC183 B2. High peak 1.09 was found after 72 hours against 0.16 in the control.The ratios of tumor tissue/non-tumor tissue 4.38 at 48 hours, 2.46 at 72 hours, but 1.11 in the control at 72 hours. The different radioactivities expressed as cpm/gm were edtermined in tumors weighing 34 mg, 70 mg, 120 mg and 400 mg respectively and the results were not very much different from each other. Nevertheless, tumor weighing less than 70 mg could not be detected by this procedure. Perhaps this is related with the sensitivity of the equipment.Obviously we have to do more work for the extrapolation from animal studies to human, however, the above results may provide valuable clues for eventual application of this procedure to patients with ovarian cancer.