UPLC-MS/MS测定中药材中19种氨基甲酸酯农药残留量

 目的建立测定中药材中19种氨基甲酸酯农药残留量的超高效液相-串联四级杆质谱方法。方法 多反应监测模式测定，以Waters ACQUITY UPLC BEH C₁₈进行分离，乙腈-0.1%甲酸溶液（1∶1，流速：0.2 mL·min-1。离子化模式：ESI+。结果 19种农药测定的线性范围在1.0～100.0 μg·L-1之间，r分别在0.994 7~0.999 7之间，检出质量浓度分别在0.02~0.46 μg·L-1之间， 3个水平的回收率分别在73.2%～119.7%之间。结论 该方法快速、准确、灵敏，可用于中药材中氨基甲酸酯类农药残留的测定。

Determination of Nineteen Carbamate Pesticides in Chinese Medicinal Materials by UPLC-MS/MS

OBJECTIVE To establish a method for the determination of 19 carbamate pesticides in Chinese medicinal materials by UPLC triple-quadrupole tandem mass spectrometer. METHODS The samples were determinated by multi-reactions monitoring (MRM technology. The separation was performed on a Waters ACQUITY UPLC BEH C₁₈ column with acetonitrile -0.1% formic acid (1∶1 with a flow rate of 0.2 mL min-1. Ion mode was set as ESI+. RESULTS The standard curves of the 19 carbamate pesticides showed good linearity over the concentration range of 1.0-100 μg·L-1 （r=0.994 7-0.999 7， respectively. The LODs of 19 carbamate pesticides were in the range of 0.02-0.46 μg·L-1 and the average recoveries were 73.2%-119.7% at three levels. CONCLUSION The method is rapid， sensitive and suitable for the determination of 19 carbamate pesticides in Chinese medicinal materials.