硫酸软骨素对慢性酒精中毒模型大鼠脑组织神经递质的影响研究

目的:探讨硫酸软骨素(CS)对慢性酒精中毒模型大鼠脑组织神经递质的影响。方法:取大鼠随机分为正常对照组、模型组、纳洛酮组(腹腔注射纳洛酮注射液,0.08mg·kg-1·d-1)和CS低、中、高剂量组(灌胃CS,分别为50、100、150mg·kg-1·d-1),每组10只。除正常照组外,其余各组灌胃给于50%乙醇溶液8mL·kg-1·d-12周后,12mL·kg-1·d-16周,建立慢性酒精中毒模型,每天给予乙醇后给予相应药物。造模8周后苏木精-伊红染色法观察各组大鼠神经组织病理学变化,并以高效液相色谱法测定各组大鼠脑组织中单胺类神经递质去甲肾上腺素(NE)、5-羟色胺(5-HT)和多巴胺(DA)的含量。结果:与模型组比较,CS低、中、高剂量组大鼠神经组织病理学损伤程度均降低,NE、5-HT、DA含量均显著降低(P<0.05或P<0.01),且中剂量组最明显。结论:CS可能通过降低慢性酒精中毒模型大鼠脑组织中单胺类神经递质的含量来减轻脑损伤。

Effects of Chondroitin Sulfate on Neurotransmitter in Brain Tissues of Rats with Chronic Alcoholism

OBJECTIVE： To study the effect of chondroitin sulfate （CS） on neurotransmitter in brain tissues of rats with chronic alcoholism. METHODS： Rats were randomly assigned to normal control group, model group, naloxone group （intraperitoneal injection of naloxone 0.08 mg·kg^-1·d^-1） and CS low-dose, medium-dose and high-dose groups （intragastric administration of CS 50, 100, 150 mg·kg^-1·d^-1） with each group of 10 rats. Except normal control group, other groups were given intragastric administration of 50% ethanol solution 8mL·kg^-1·d^-1 for 2 weeks. After that those groups were given intragastric administration of 50% ethanol solution 12 mL·kg^-1·d^-1 for 6 weeks to establish chronic alcoholism model. Model rats were treated with relevant drugs after giving ethanol. 8 weeks after modeling, HE-staining was used to observe the pathological change of nervous tissue. The content of NE, 5-HT and DA in brain tissues were determined by HPLC. RESULTS： Compared with model group, the pathological change of nervous tissue in CS low-dose, medium-dose and high-dose groups decreased. The content of NE, 5-HT and DA in brain tissues decreased significantly （P〈0.05 or P〈0.01）, especially in CS medium-dose group. CONCLUSION： CS could decrease the con- tent of neurotransmitter in brain tissues of rats with chronic alcoholism to reduce brain damage.