苯扎溴铵的体外杀精效果及对阴道粘膜刺激性研究

目的 :研究苯扎溴铵的体外杀精效果及其对大鼠阴道粘膜的刺激性 ,以初步评价该药作为外用避孕药应用的可能性。方法 :将药物溶液与新鲜精液直接混合后镜检 ;用导管将药物凝胶涂布于大鼠阴道内 ,每天一次 ,一周后解剖做病理切片观察并评分。结果 :苯扎溴铵的最低杀精浓度为 0 .2 7mg/m L,略高于壬苯醇醚 - 9(0 .2 0 mg/m L ) ;苯扎溴铵凝胶对大鼠阴道粘膜刺激性评分值为 7.6 7±0 .82 ,在可接受的范围内 ,与壬苯醇醚 - 9组 (7.3 3± 0 .82 )相比无统计学差异。结论 :苯扎溴铵具有体外杀精作用 ,但同时也有轻度、可接受的阴道刺激性 ,作为外用避孕药是可行的

Spermicidal Effect of Benzalkonium Bromide in Vitro and Its Irritative Effect on Rats' Vagina

Objective:To evaluate the spermicidal effect of Benzalkonium Bromide in vitro and its vaginal irritative effect on rats. Methods: To observe the spermic activity by using the microscope after mixing fresh sperm with Benzalkonium Bromide, we had daubed the rat's vagina with Benzalkonium Bromide Gel once a day for seven days, and then anatomised rats, obtain effective the vaginal tissue and observed the pathologic slices. Results: The minimal concentration of Benzalkonium Bromide was 0.27 mg/mL, slightly higher than that of wonoxynol-9 (0.20 mg/mL). Following the accepted Eckstein meteyard, the effect score of Benzalkonium Bromide gel's on rats' vagina was 7.67±0.82, and by compared with Nonoxynol-9 gel's score(7.33±0.82), there is no significant difference in the result of t-test. Conclusion: It is feasible to using Benzalkonium Bromide as a spermicide.