黄竹清脑颗粒对痰热腑实证脑出血大鼠IL-6、Na^＋-K^＋-ATP酶活性及脑含水量的影响

目的：探讨黄竹清脑颗粒对痰热腑实型脑出血大鼠模型血清中IL-6、Na^＋-K^＋-ATP酶的活性及脑含水量的影响。方法：采用脑内注射胶原酶I-肝素混合液配合大鼠自体粪便灌胃的方法建立大鼠脑出血痰热腑实证模型，将SD大鼠随机分为空白对照组、黄竹清脑颗粒大、中、小剂量组、二陈汤提取液组、鲜竹沥口服液组、痰热腑实证模型组，分别采用放免法及光化学比色法测定各组大鼠血清中IL-6、脑组织匀浆中Na^＋-K^＋-ATP酶的活性及脑组织含水量的变化。结果：（1）模型组与空白组相比，血清中IL-6水平及脑组织含水量明显升高（P〈0．01），脑组织中Na^＋-K^＋-ATP酶明显降低（P〈0．01）；（2）黄竹清脑颗粒大、中剂量组疗效均明显优于二陈汤组、鲜竹沥组及黄竹清脑颗粒小剂量组。结论：黄竹清脑颗粒可显著提高脑组织中Na^＋-K^＋-ATP酶的活性，降低血清中IL-6的含量，具有减轻细胞内水肿、抑制炎性反应，保护脑神经细胞的作用；并对痰热腑实型脑出血有明显的改善作用。

Effect of Granule of Whangee Clearing Mind on IL-6, Na +-K +-ATP Enzyme and Brain Moisture Capacity in Rats with Brain Hemorrhage Belonging to Phlegm-heat and FU-organ Sthenia Syndrome

Objective: To investigate the effect of Granule of Whangee Clearing Mind on IL-6, Na + -K + -ATP enzyme and brain moisture capacity in rats with brain hemorrhage belonging to phlegm-heat and FU-organ sthenia syndrome. Method : The model of phlegm-heat and FU-organ sthenia syndrome was made by injecting mixed liquor of clostridiopeptidase Ⅰ-calparine and autos dejecta to brain. SD rats were divided into blank control group, large dose group of Granule of Whangee Clearing Mind, middle close group group of Granule of Whangee Clearing Mind, low dose group of Granule of Whangee Clearing Mind, Er-chen Decoction extract group, bamboo juice group and model group. Determine IL-6 in serum, Na + -K + -ATP enzymatic activity in brain homogenate and brain moisture model group increased more obviously than in blank control group ( P < 0.01 ), and Na + -K + -ATP enzyme de- Whangee Clearing Mind were better remarkably than Er-chen Decoction extract group, bamboo juice group and low dose group of Granule of Whangee Clearing Mind. Conclusion: Granule of Whangee Cleating Mind can improve Na+ -K+ -ATP enzymatic activity and cut down IL-6, has the effects of lessening intracellular edema, inhibiting in- flammatory reaction and protecting cerebral neurocyte. At the same time it can cure brain hemorrhage belonging to phlegm-heat and FU-organ sthenia syndrome.