Hsp70-肿瘤抗原肽复合物防治小鼠黑色素瘤B16肺转移的作用

目的:探讨热休克蛋白70-肿瘤抗原肽复合物(Hsp70-antigen peptide complexes)对小鼠黑色素瘤B16转移的防治作用.方法:分别从小鼠腿部接种的B16实体瘤及小鼠肺B16转移灶提取混合抗原肽,体外与Hsp70结合制得复合物,此复合物免疫小鼠后用于预防或治疗经尾静脉接种转移至肺的B16黑色素瘤,观察其对肿瘤转移的防治作用.结果:Hsp70-肿瘤抗原肽复合物免疫后肺转移灶节结数显著减少(P＜0.01),体外脾细胞表现出对B16较高的杀伤率(P＜0.01);并对肺转移灶有显著的治疗作用(P＜0.01),而从B16实体瘤提取的混合抗原肽制得的复合物比从肺转移灶提取的混合抗原肽制得复合物有更好的治疗效果(P＜0.01),表现出体外脾细胞对B16更高的杀伤率(0.01＜P＜0.05).结论:Hsp70-肿瘤抗原肽复合物对肿瘤的转移有明显的防治作用,而从实体瘤提取的混合抗原肽比从转移灶提取的混合抗原肽更为有效.

The Prevention and Therapeutic Effects of Hsp70-Antigen Peptide Complexes on B16 Tumor Pulmonary Metastasis in Mice

Objective: To explore the preventive and therapeutic effects of Hsp70-antigen peptide complexes (HACs) on pulmonary metastasis of malanoma B16 cells in mice. Methods: Antigen peptide mixtures were prepared from massive tumors and metastatic foci respctively, and bound with Hsp70 in vitro. The HACs were used to mice to prevent the formation of micrometastatic foci after the injection of B16 cells through tail vein, or treat the existent micrometastatic foci in lung The number of metastatic foci was counted and the cytotoxicity of splenocytes to B16 cells was determined. Results: After immunization with HACs, the number of metastatic foci in mice decreased significantly (P <0. 01) , and the cytotoxicity of splenocytes to B16 cells was signficantly enhanced (P < 0. 01) . The immuniation with HACS can both prevent the formation of metastatic foci and freat the existent metastatic foci. The immunization with petide mixture from massive tumros produced better therapeutic effect ( P < 0. 01) , and induced stronger citotoxicity of splenocytes to B16 cells (0. 01