| 对钒酸氧化法测定血清中胆红素的评价 |
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| 引用本文： | 沈瑛,刘茜,刘巍,宋克征.对钒酸氧化法测定血清中胆红素的评价[J].医学检验与临床,2003,14(5):4-6. |
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| 作者姓名： | 沈瑛 刘茜 刘巍 宋克征 |
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| 作者单位： | 1. 中国医学科学院,协和医科大学,北京协和医院检验科,北京,100730
2. 北京潞河医院检验科,北京,100730 |
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| 摘 要： | 目的:评价钒酸氧化法测定血清中总胆红素和直接胆红素。方法:钒酸氧化法的精密度、准确度实验及其与重氨法相关和干扰因素实验。结果:批内CV总、直接胆红素分别为0.8％与1.3％。日间CV为1.8％与1.7％,平均回收总胆红素为101.1％,直接胆红素100.1％,与重氮试剂法相关(J-G法)总胆为y(本法)=1.015x(重氮法)-0.119,r=0.998,直接胆红素为y(本法)1.101x(重氮法)+0.81,r=0.952。相关良好,血红蛋白浓度和脂血分别高达3g/L和9.0mmol/L不干扰。结论:该法简单、快速、稳定,而且对溶血、乳糜等干扰因素的影响比重氮法小。
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| 关 键 词： | 胆红素 钒酸氧化法 |
Oxidation Method of Measuring Bilirubin in Serum by Vanadic acid |
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| Shen Ying,Liu Qian,Liu Wei,Song Kezheng.Oxidation Method of Measuring Bilirubin in Serum by Vanadic acid[J].Medical Laboratory Science and Clinics,2003,14(5):4-6. |
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| Authors: | Shen Ying Liu Qian Liu Wei Song Kezheng |
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| Institution: | ShenYing Liu Qian Liu Wei Song
Kezheng (PUMC Hospital Beijing 100730) (Luhe hospital) |
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| Abstract: | Objective We introduce Oxidation method of measuring bilirubin in serum by Vanadic acid. Methods The acuraey precision as well as interferencce experiment of vanadic have been made. The correlation analysis of vanadic and Diazo methods also have been made. Results The CVs of determination total and direct bilirubin were 0.8% and 1.3% for intra- run, 1.8% and 1.7% for between day respectively. The average recovery was 101.1% for total bilirrbin, and 100.1% for direct bilirubin. The result of the method correlation well with Diazo method.Their's regression equation and r value were,TBIL y(Vanadate) = 1. 105x (Diazo) -0.119,r = 0.998, DBIL y( Vanadate) = 1. 101 X (Diazo) + 0.81 r = 0.952. Conclusion The method is single, rapid and stable. It has the ability to anti - interference of hemoglobin and chy-lomicronemia. |
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| Keywords: | Bilirubin Vanadio acid Oxidation method |
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