Calbindin-D28k与肌动蛋白在人健康及龋坏牙齿中的分布

目的 探讨calbindin—D28k与肌动蛋白在健康和龋坏人恒牙中的分布相关性及意义。方法 用免疫组化方法分别检测calbindin—D28k与肌动蛋白在正常、中龋和深龋人牙中的表达并观察比较。结果 正常组Calbindin—D28k在前期牙本质远髓2／3区域染色阳性，肌动蛋白在成牙本质细胞及住于矿化牙本质中的细胞突呈阳性；中龋组位于前期牙本质中的成牙本质细胞突Calbindin—D28k与肌动蛋白均呈强阳性，Calbindin—D28k在病损区前期牙本质全层呈阳性且较正常组明显增强；深龋组Calbindin—D28k染色部位主要位于成牙本质细胞核。结论 在第三期牙本质形成活跃的成牙本质细胞中Calbindin—D28k与肌动蛋白的分布变化具有一致性，均与牙本质基质的分泌有关。

Distribution of Calbindin-D28k and actin in healthy and carious teeth

Objective To investigate the distribution of Calbindin-D28k and actin in healthy and carious teeth.Methods Calbindin-D28k and actin were Examined by immunochemical technique and their distributions in normal,intermediate and deep carieswere compared.Results In the normal teeth, Calbindin-D28k was distributed in the distal 2/3 region of the predentin while actin was present in the odontoblasts and their processes extending into the mineralized dentin.In the intermediate caries,the odontoblast processes within the predentin were strongly labeled for both Calbindin-D28k and actin,and Calbindin-D28k was found throughout the predentin area and became much stronger.In the deep caries,Calbindin-D28k was detected mainly in the nucleus of the odontoblast.Conclusion Calbindin-D28k and actin were concordant in the odontoblasts under active tertiary dentin formation,suggested that both two proteins could be associated with the secretion of dentin matrix.