不同脱乙酰度壳聚糖支架制备及降解性能评价

制备不同脱乙酰度壳聚糖支架,采用SEM观察其表面形貌,检测孔隙率,吸水溶胀率,体内、外降解率。结果表明不同脱乙酰度支架均具有高孔隙三维结构。随脱乙酰度增加,孔隙率分别为93.46%、90.02%和86.71%;溶胀率分别为820%、803%和772%;第4周体外降解率分别为30.44%、22.88%和17.10%;体内降解率为57.48%、40.23%和29.53%。其降解率与脱乙酰度呈负相关,体内降解速率快于体外。可通过控制壳聚糖的脱乙酰度大小为软骨缺损修复提供匹配良好的降解支架材料。

Preparation of chitosan scaffold with different deacetylated degrees and evaluation of the degradation characteristics

The chitosan scaffolds with different deacetylated degree were prepared in this study. The morphology of scaffolds were observed using SEM, and the porosity, the water absorbing swelling ratio and the degradation were examined both in vitro and in vitro. The results showed that the chitosan scaffolds with different deacetylated degree exhibited three-dimensional structure with high porosity. With increasing of deacetylated degree, their porosities were 93.46%, 90.02% and 86.71%, respectively. The swelling ratios of chitosan scaffolds were 820%, 803% and 772%, respectively. At the fourth week, the degradation rates were 30.44%, 22.08% and 17.10% in vitro, respectively; while the corresponding rates were 57.48%, 40.23%, 29.53% in vivo respectively. The degradation rate of chitosan scaffold was negatively correlated to deacetylated degree. Furthermore, it showed that the speed of degradation in vivo was faster than that in vitro. We concluded that controlling the deacetylated degree of chitosan can provide a well-matched degradable scaffold material for the reparation of cartilage defects.