A method for modifying the adhesive phenotype of wild isolates ofEscherichia coli in coli-bacterin producer strains |
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| Authors: | E. V. Sokurenko V. L. Chesnokova |
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| Affiliation: | (1) Department of Biomedical Technology, I. M. Sechenov Moscow Medical Academy, moscow;(2) Department of Anatomy and Neurobiology, University of Tennessee, Memphis, USA |
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| Abstract: | Use of the suicidal vector pCH103 permitted the creation of recombinant strain with the chromosome DNA site coding for adhesin (FimH) replaced by the kanamycin resistance gene (npt). The resultant chimeras transformed by vectors containingfimH genes of different origin express type I piles of the relevant phenotype. This method was used to modifyE. coli M17 producer strain with an unfavorable adhesive phenotype. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 124, No. 9, pp. 334–338, September, 1997 |
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| Keywords: | coli-bacterin adhesion adhesive phenotype |
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