胰岛素样因子3在氟他胺诱导的小鼠隐睾模型中的表达和意义

目的:通过氟他胺诱导小鼠隐睾动物模型的建立,探讨其睾丸组织的胰岛素样因子3(INSL-3)mRNA表达的变化。方法:妊娠BALB/c小鼠随机分成A、B、C、D、E 5组,每组9只(雄鼠3只,雌鼠6只),合笼交配;分别在雌鼠孕第12²1天持续10 d给予氟他胺灌胃,剂量依次为0、150、300、500、700 mg/kg;分别在出生后第4周和第8周时运用荧光定量PCR技术检测子代小鼠睾丸组织中INSL-3 mRNA的表达情况。结果:A组小鼠无隐睾,B、C组有单侧隐睾,发生率分别10.0%和25.0%;D、E组有双侧隐睾的发生,发生率分别为21.1%和40.0%。随着给药剂量的增大,INSL-3 mRNA的表达逐渐降低;与对照组相比,B、C组表达强度无明显变化(P>0.05);D、E组表达强度明显低于A组(P<0.05)。结论:剂量为150与300 mg/kg的氟他胺能诱导单侧隐睾模型,剂量为500与700 mg/kg的氟他胺能诱导双侧隐睾模型;并且隐睾小鼠睾丸的INSL-3表达随氟他胺浓度升高水平明显下降,可能是隐睾发生的机制之一。

Expression of insulin-like factor 3 in the testis of flutamide-induced cryptorchidism mice and its significance

[ Abstract] Objective ： To study the changes in the mRNA expression of insulin-like factor 3 （ INSL-3 ） in the testis of mouse models of flutamide-induced cryptorchidism. Methods： We randomized pregnant BALB/c mice to groups A （control） , B, C, D and E to receive continuous gavage of flutamide at 0, 150, 300, 500 and 700 mg/kg body weight, respectively, from gestation day 12 to 21. We detected the expression of INSL-3 mRNA in the testis of the neonates by real-time PCR at 4 and 8 postnatal weeks. Results ： No cryptorchidism was found in group A; unilateral cryptorchidism was seen in groups B （ 10.0% ） and C （25.0%） ; and bilateral cryptorchidism was observed in groups D （21.1% ） and E （40.0%）. The expression of INSL-3 mRNA was reduced with the increased dose of flutamide, not significantly changed in groups B and C （P 〉 0.05 ） but remarkably decreased in D and E as compared with A （P 〈00. 05）. Conclusion： Administration of flutamide to pregnant mice can induce unilateral cryptorchidism at 150 and 300 mg/kg and bilateral cryptorehidism at 500 and 700 mg/kg in their male offspring, which may be related with its reducing effect on the expres- sion of INSL-3 in the testis of the mice. Natl J Androl, 2013, 19 （11） ： 968 -971