酶联免疫吸附试验测定子宫内膜IgG抗体诊断子宫内膜异位症价值的评价

目的评价酶联免疫吸附试验(ELISA)测定子宫内膜IgG抗体诊断子宫内膜异位症(endometriosis，EMs)的临床价值。方法采集空腹血清91份，分三组，EMs组39例，正常对照组15例，良性疾病对照组37例，用酶联免吸附法对三组血清中子宫内膜IgG抗体进行测定。结果EMs组阳性率76.92％，正常对照组阳性率(33.33％)与EMs组比较有显著性差异(P＜0.005)，疾病对照组阳性率(62.16％)与EMs组比较差异不明显(P＞0.05)。ELISA法测定血清子宫内膜IgG抗体诊断EMs敏感性76.92％，特异性46.15％。结论ELISA法虽敏感、有效、简单，但不能作为一种最有价值的诊断EMs的辅助方法。

Value of endometrial immunogiobulin G antibodies measured by enzyme-linked immunosorbent assay in diagnosing endometriosis.

Objective To investigate the clinical value of diagnosing endometriosis(EMs)by measuring endometrial immunoglobulin G antibodies with enzyme linked immunosorbent assay(ELISA).Methods The sera(n=91)were divided into EMs group(n=39),normal(n=15)and disease(n=37)control groups,and ELISA was used to measure endometrial immunoglobulin G antibodies in sera from each group.Results The positive rate of endometrial immunoglobulin G antibodies in EMs group(76^92%)was significantly higher than that in normal control group 33^33%(P<0^005),but no significant difference was observed between EMs and disease control group(P>0^05).The experiment's sensitivity and specificity were 76^92% and 46^15% respectively.Conclusion Although measuring endometrial immunoglobulin G antibodies with ELISA is simple,sensitive and efficient,it can't act as the most valuable method in diagnosing endometriosis.