丁癸草的异黄酮类化学成分研究

 目的 研究丁癸草的化学成分。方法 通过硅胶柱色谱、Sephdex LH20凝胶柱色谱、反相硅胶C-18及高压液相色谱等方法对丁癸草进行分离，运用现代光谱技术鉴定化合物结构；并通过MTT法对其抑制人食管癌细胞TE13和小鼠黑色素瘤细胞B16的增殖进行了研究。结果 从丁癸草中分离得到6个具有抗肿瘤作用的异黄酮类化合物，分别鉴定为7，4′-二甲氧基异黄酮（1）；7-羟基-4′-甲氧基异黄酮（2）；7，3′-二羟基-4′-甲氧基异黄酮（3）；7，8-二羟基-4′-甲氧基异黄酮（4）；7，4′-二羟基-8-甲氧基异黄酮（5）；7，4′-二羟基异黄酮（6）。结论 所有化合物均首次从丁癸草植物中分离得到，并且具有抑制人食管癌TE13细胞的作用，其中化合物3对小鼠黑色素瘤B16细胞的增殖也有抑制作用。

Study on Chemical Constituents of Zornia diphylla

OBJECTIVE To investigate the chemical constituents of Zornia diphylla(L.) Pers.METHODS The compounds were isolated by chromatography on silica gel,Sephdex LH20,ODS C-18 and HPLC,and their structures were elucidated on the basis of spectral analysis.MTT assay in vitro was used to explore the inhibiting effect of the compounds on TE13 cancer cells and B16 cancer cells.RESULTS Six compounds were isolated and identified as 7,4′-dimethoxy isoflavone(1);7-hydroxy-4′-methoxyisoflavone(2);7,3′-dihydroxy-4′-methoxyisoflavone(3);7,8-dihydroxy-4′-methoxyisoflavone(4);7,4′-dihydroxy-8-methoxyisoflavone(5);and 7,4′-dihydroxy-isoflavone(6).All compounds showed anti-tumor activities against the cancer cells.CONCLUSION All compounds are isolated from Zornia diphylla(L.) Pers.for the first time.Compounds 1-6 show inhibitory activity on TE13 cancer cells,and compound 3 shows inhibitory activity on B16 cancer cells.