| 鼠源抗胃癌抗体轻链可变区基因克隆及其结构分析 |
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| 引用本文： | 李陕区,张康泰,解放军,闫小君. 鼠源抗胃癌抗体轻链可变区基因克隆及其结构分析[J]. 医学争鸣, 2000, 21(5): S095-S097 |
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| 作者姓名： | 李陕区 张康泰 解放军 闫小君 |
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| 作者单位： | 1. 第四军医大学基因诊断技术研究所,陕西,西安,710033
2. 解放军451医院医教部 |
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| 摘 要： | 目的 扩增与人胃癌细胞特异结合的鼠源性抗体轻链可变区基因，并对其基因序列进行分析。方法 用ＲＴ－ＰＣＲ方法从分泌鼠源抗人胃癌抗体的杂交瘤细胞扩增出抗体轻链可变区基因，克隆到ｐＧＥＭ－Ｔ载体，测序仪测定其基因序列并对其结构进行分析。结果 所获得的基因由３５４ｂｐ组成，编码１１８个氨基酸，序列分析证实该基因可变区序列符合小鼠Ｉｇ轻链可变区的氨基酸序列特征。结论 获得的鼠源抗胃癌抗体轻链可变区基因为ｋⅢ
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| 关 键 词： | 胃肿瘤 抗体 杂交瘤细胞 克隆 |
Cloning and constructive analysis of mouse anti-gastric-cancer antibody light chain variable region gene fragments |
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| LI ShanQu,ZHANG KangTai,YAN XiaoJun Institute of Gene Diagnostic Technique of Chinese PLA,Fourth Military Medical University,Xi'an ,China,Administration of Medicine and Education,Chinese PLA st Hospital. Cloning and constructive analysis of mouse anti-gastric-cancer antibody light chain variable region gene fragments[J]. Negative, 2000, 21(5): S095-S097 |
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| Authors: | LI ShanQu ZHANG KangTai YAN XiaoJun Institute of Gene Diagnostic Technique of Chinese PLA Fourth Military Medical University Xi'an China Administration of Medicine Education Chinese PLA st Hospital |
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| Affiliation: | LI ShanQu1,ZHANG KangTai2,YAN XiaoJun1 1Institute of Gene Diagnostic Technique of Chinese PLA,Fourth Military Medical University,Xi'an 710033,China,2Administration of Medicine and Education,Chinese PLA 451st Hospital |
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| Abstract: | |
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| Keywords: | gastricneoplasm antibody hybridoma cell clone |
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