银杏叶提取物对兔动脉粥样斑块Bcl-2和Bax表达的影响

目的 观察银杏叶提取物对家兔动脉粥样斑块Bcl-2和Bax表达的影响.方法 将40只家兔随机分为正常对照组、模型组、立普妥组和银杏叶提取物组,对比观察各组家兔斑块面积大小及斑块Bax和Bcl-2的表达.结果 银杏叶提取物组和立普妥组动脉粥样斑块面积低于模型组(P<0.01);免疫组织化学染色显示,模型组平滑肌细胞内Bcl-2和Bax的阳性细胞表达与正常对照组相比增高(P<0.05),立普妥组和银杏叶提取物组平滑肌细胞、泡沫细胞Bax的阳性细胞表达下调,Bcl-2的阳性细胞表达轻度上调,银杏叶提取物组Bax/Bcl-2降低.结论 银杏叶提取物可通过下调Bax/Bcl-2表达而抑制细胞凋亡,减少内皮细胞受损,从而抑制动脉粥样硬化的发生.

Effects of Ginkgo Biloba Extract on the Expression of Bcl-2 and Bax in the Rabbit Atherosclerotic Plaque

Aim To observe the Ginkgo biloba extract(EGB) on Bcl-2 and Bax expression of rabbit atherosclerotic plaques. Methods 40 rabbits were randomly divided into untreated control group,model group,lipitor group and EGB group.The changes of atherosclerotic plaque area and the expressions of Bcl-2 and Bax were observed. Results The plaque area of EGB group and lipitor group were less than those of the model group(P><0.01).The positive expressions of Bcl-2 and Bax in model group were higher than those in untreated control group(P><0.05),the positive expressions of Bax of smooth muscle cells and foam cells decreased in lipitor group and EGB group,the positive expressions of Bcl-2 increased,furthermore Bax/Bcl-2 in EGB group decreased. Conclusion EGB can decrease the expression of Bax/Bcl-2,reduce endothelial cell damage,thus inhibiting the occurrence of atherosclerosis.