透皮吸收促进剂对大黄中蒽醌类有效成分透皮吸收的影响

目的 探讨不同浓度的氮酮、丙三醇、油酸对大黄中蒽醌类有效成分透皮吸收的影响. 方法以离体小鼠皮为透皮屏障,采用改进Franz扩散池装置,采用高效液相色谱法同时测定接收池中蒽醌类物质大黄素,大黄酚,大黄素甲醚,大黄酸的含量,计算累积透过量.结果 氮酮、丙三醇和油酸对大黄中蒽醌类物质均有促透作用,其促透作用强弱顺序为:2%氮酮>1%氮酮>4%氮酮>6%氮酮;6%丙三醇>4%丙三醇>2%丙三醇>1%丙三醇;2%油酸>4%油酸>6%油酸>1%油酸. 结论氮酮、丙三醇和油酸对大黄中蒽醌类物质大黄素、大黄酚、大黄甲醚、大黄酸的促透作用具有一定的量效关系.

Effects of Skin Penetration Enhancers on the Transdermal Absorption of Effective Components in Anthraquinones from Rhubard

Objective To evaluate the effect of Azone, propanetriol and oleic acid under different concentrations on Transdermal absorption of effective components in anthraquinones from Rhubard. Methods A piece of excised abdominal hairless mouse skin was set in a Franz-type diffusion cell.Penetrations of emodin, rhein, physcion and chrysophanol were detected by HPLC and cumulative permeation(Q) calculated.Results Laurocapram, propanetriol and oleic acid were found permeation enhancing effect to anthraquinones (emodin, rhein, physcion and chrysophanol) in Rhubard. The permeation enhancing effect was 2% oleic acid >4% oleic acid >6%oleic acid >1% oleic acid;2%laurocapram >1% laurocapram >4% laurocapram >6% laurocapram;6% propanetriol >4% propanetriol >2% propanetriol >1% propanetriol. Conclusion Laurocapram, propanetriol and oleic acid have permeation enhancing effect to anthraquinones (emodin, rhein, physcion and chrysophanol) in Rhubard with dose-effect relationship.