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| Alteration of Levels of Cyclic Nucleotide and Analysis of SDS－PAGE in Autologous Transplanted Spleen And Splenic Artery Ligat | |
| 引用本文： | 汤绍涛,阮庆兰,郭筱兰,叶明,周晓初. Alteration of Levels of Cyclic Nucleotide and Analysis of SDS－PAGE in Autologous Transplanted Spleen And Splenic Artery Ligat[J]. 华中科技大学学报(医学英德文版), 1996, 16(3): 160-163. DOI: 10.1007/BF02908798 |
| 作者姓名： | 汤绍涛 阮庆兰 郭筱兰 叶明 周晓初 |
| 作者单位： | Department of Pediatric Surgery，Xiehe Hospital，Tongji Medical University，Wuhan 430022 |
| 摘 要： | Ａｌｔｅｒａｔｉｏｎｏｆ ＬｅｖｅｌｓｏｆＣｙｃｌｉｃＮｕｃｌｅｏｔｉｄｅａｎｄ ＡｎａｌｙｓｉｓｏｆＳＤＳ－ＰＡＧＥｉｎＡｕｔｏｌｏｇｏｕｓＴｒａｎｓｐｌａｎｔｅｄＳｐｌｅｅｎＡｎｄ ＳｐｌｅｎｉｃＡｒｔｅｒｙＬｉｇａｔｉｏｎＴＡＮＧＳｈａｏｔａｏ... |
Alteration of levels of cyclic nucleotide and analysis of SDS-PAGE in autologous transplanted spleen and splenic artery ligation |
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| Tang Shaotao,Ruan Qinglan,Guo Xiaolan,Ye Ming,Zhou Xiaochu. Alteration of levels of cyclic nucleotide and analysis of SDS-PAGE in autologous transplanted spleen and splenic artery ligation[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 1996, 16(3): 160-163. DOI: 10.1007/BF02908798 | |
| Authors: | Tang Shaotao Ruan Qinglan Guo Xiaolan Ye Ming Zhou Xiaochu |
| Affiliation: | 1. Department of Pediatric Surgery, Xiehe Hospital, Tongji Medical University, 430022, Wuhan |
| Abstract: | Intracellular levels of cyclic nucleotide were detected and mitogen stimulation assays were performed in young Sprague-Daewley rats and suspension of spleen tissue were separated by SDS-PAGE. Results indicated that intracellular levels of cyclic AMP in transplanted cell were significantly lower compared to the control group(P<0.01), the levels of cyclic GMP in transplant cell and the levels of cyclic nucleotide in splenic artery ligation groups were normal. Immunologic tests showed that the stimulation index by Con A for T cells was drastically decreased in the autotransplant and a normal proliferation of B cells after LPS stimulation in transplants. Electrophoresis showed differences in the protein patterns between both tissues. Mitogen stimulation and the protein patterns were not different between the control and splenic artery ligation groups. There were differences between the normal tissues and the transplants at the functional level, Suggesting simple autotransplant can not prevent overwhelming postsplenectomy infection. The intact cellular function after splenic artery ligation indicated that its anti-infection ability is superior to that of splenic transplants. |
| Keywords: | autotransplantation splenic artery ligation cyclic nucleotide mitogen stimutation SDS-PAGE |
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