胃癌与MLVD的相关性研究

目的:检测胃癌患者肿瘤组织微淋巴管密度(micro-lymphatic vessel density,MLVD)的表达,分析MLVD与年龄、性别、肿瘤组织分化、淋巴结转移、分期等的关系,探讨抗淋巴管生成治疗的可能性。方法:收集胃癌术后标本及同一患者肿瘤边缘和距离肿瘤5 cm以上的正常组织标本,采用免疫组化方法测定肿瘤组织、肿瘤边缘和正常胃组织MLVD值,并结合临床及病理资料,分析MLVD值与年龄、性别、肿瘤组织分化、淋巴结转移、分期等的关系。结果:胃癌边缘组织MLVD显著高于正常组织及胃癌组织。胃癌边缘组织MLVD与胃癌的分期显著相关(P<0.01)。胃癌边缘组织MLVD与淋巴结转移相关(P<0.05),但与年龄、性别、肿瘤组织分化无明显相关性(P>0.05)。胃癌组织MLVD与胃癌的分期相关(P<0.05)。胃癌组织MLVD与淋巴结转移相关(P<0.05)。但与年龄、性别、肿瘤组织分化无明显相关性(P>0.05)。结论:胃癌组织边缘的MLVD显著高于胃癌组织及正常组织,且与淋巴结转移、临床分期有关,其MLVD的增加可能与胃癌的侵袭及转移相关。胃癌组织中MLVD的检测可作为评估胃癌淋巴结转移、判断胃癌生物学行为及预后的指标之一。

Relationship of gastric cancer and MLVD

Objective:To detect the amount of MLVD(micro-lymphatic vessel density) in gastic cancer,analyze the relationship of MLVD with the following factors: age,gender,clinical stage,lymph node metastasis,histo-differentiation.Methods: Gastric cancer tissue and bordeline tissue of cancer and normal tissue at least 5cm away from the tumor were collected.We adopted an immunohistochemistry method to determine the level of MLVD in the samples.Results: MLVD in tumor borderline was higher than that in the normal tissue and gastric cancer(P<0.01).MLVD in tumor borderline had positive relation to the clinical stage(P<0.01).MLVD in tumor borderline had positive relation to lymph node metastasis(P<0.05).But it had no significantly difference in age,gender and cancer histo-differentiation(P>0.05).MLVD in tumor tissue had positive relation to clinical stage(P<0.05)and lymph node metastasis(P<0.05).But it had no significantly difference with age,gender and cancer histo-differentiation(P>0.05).Conclusion: MLVD in tumor borderline was significantly higher than in gastric cancer and normal tissue,and has correlation with clinical stage,lymph node matastasis.The increase of MLVD may be related to invasion and metastasis of gastric cancer in tumor borderline.The detection of MLVD in the tissue of gastric cancer can be accepted as important affiliated marker to evaluate lymph node metastasis.It may be marker to predict the malignant biology of gastric cancer and prognosis.