三维支架与肋软骨细胞体外及体内培养构建组织工程化气管

目的 探索建立适合替代气管的组织工程化气管模型.方法 分离2月龄新西兰兔肋软骨细胞并传代二次后,以5×107/ml浓度分别种于PLGA和涤纶支架,体外环境中培养2周.将支架-软骨细胞模型植入裸鼠脊柱两侧皮下,培养4、6、8周后取出,HE染色、PAS染色、扫描电镜等观察软骨组织发育、新生毛细血管、整体组织结构层次.结果 种植软骨细胞的支架经体外和体内培养,软骨细胞及基质生长分泌旺盛,支架与上皮间存在紧密的纤维连接,含有丰富的新生毛细血管.涤纶-软骨细胞模型和PLGA-软骨细胞模型可以形成完整的组织结构.结论 肋软骨细胞种植于PLGA和涤纶支架材料上,经体外及体内培养构建的组织工程化气管模型符合气管替代要求.

Abstract：

Objective To investigate the effect of a tissue engineered trachea for replacement fabricated using three dimensional scaffold and chondrocytes by in vitro and in vivo culturing. Methods Rib chondrocytes were isolated and expanded to two passages, then seeded in PLGA or Dacron scaffold at density of 5 × 107/ml. Cultured in vitro for two weeks, the chondrocytes-scaffold model was planted under dorsal skin between nude mice's spine. Histology of cartilage, neovascularization and organizational structure were observed with HE staining, PAS staining and electron microscopic scan were performed after 4,6,8 weeks in vivo. Results Organized structure were observed in both PLGA-chondrocyte model and dacron-chondrocyte model with cartilage formation, neovascularization and tight fibrous connective tissue between scaffold and skin after in vitro and in vivo culture. Conclusion Tissue engineered trachea fabricated using rib chondrocytes and PLGA or dacron scaffold with in vitro and in vivo culture meets the requirement of trachea replacement.

Fabrication of tissue engineered trachea by in vitro and in vivo culturing of chondrocytes in plga or dacron porous scaffolds

Objective To investigate the effect of a tissue engineered trachea for replacement fabricated using three dimensional scaffold and chondrocytes by in vitro and in vivo culturing. Methods Rib chondrocytes were isolated and expanded to two passages, then seeded in PLGA or Dacron scaffold at density of 5 × 107/ml. Cultured in vitro for two weeks, the chondrocytes-scaffold model was planted under dorsal skin between nude mice's spine. Histology of cartilage, neovascularization and organizational structure were observed with HE staining, PAS staining and electron microscopic scan were performed after 4,6,8 weeks in vivo. Results Organized structure were observed in both PLGA-chondrocyte model and dacron-chondrocyte model with cartilage formation, neovascularization and tight fibrous connective tissue between scaffold and skin after in vitro and in vivo culture. Conclusion Tissue engineered trachea fabricated using rib chondrocytes and PLGA or dacron scaffold with in vitro and in vivo culture meets the requirement of trachea replacement.