顺式作用元件的预测

顺式作用元件是同一DNA分子中具有特殊功能的转录因子DNA结合位点和其它调控基序,在基因转录起始调控中起重要作用;按功能特性分为通用调节元件如启动子、增强子及沉默子和专一性元件如激素反应元件,cAMP反应元件;确定顺式作用元件的试验方法主要有:DNA结构分析、序列分析和基因删除或替换等,软件预测是确定顺式作用元件的另一种方法,但不同软件预测结果差异较大,将试验方法与软件预测相结合能够提高顺式作用元件预测的准确性。

Prediction of cis-Acting Regulatory Elements

Cis-acting regulatory elements,including general regulatory elements such as promoter,enhancer and silencer and special response elements such as cAMP response elements,hormone response elements,are specifically functional short DNA sequence located in the same gene.Over the past decades,much progress has been made in predicting cis-acting regulatory elements by experimental methods: DNA structure and sequence analysis,DNA deletion mutagenesis,and prediction software.Although the two methods have some limitations,combination use of them could enhance the efficiency in finding cis-acting elements of unknown DNA sequence.