A one-step procedure for preparation of classical pathway (C1q) and alternative pathway (factor D) depleted human serum |
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| Authors: | F. Praz M.C.Roque Antunes Barreira P. Lesavre |
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| Affiliation: | Clinique Néphrologique and INSERM U-25, Hôpital Necker, 161, rue de Sèvres, 75730 Paris Cedex 15, France |
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| Abstract: | A simple method for preparation of a serum depleted in both C1q and Factor D is described. The hemolytic activities of both pathways are completely abolished and can be fully restored using the respective purified complement components. Furthermore, this serum is useful for studying cell systems since blocking either complement pathway does not require chelating agents. |
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| Keywords: | complement alternative pathway classical pathway hemolytic assay complement technology |
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