|
|||||
|
|
Paternity testing after pregnancy termination using laser microdissection of chorionic villi |
|
| Authors: | M Bauer Andreas Thalheimer Dieter Patzelt |
| Institution: | (1) Institute of Legal Medicine, University of Würzburg, Versbacher Strasse 3, 97078 Würzburg, Germany e-mail: reme005@mail.uni-wuerzburg.de, DE;(2) Institute of Pathology, University of Würzburg, Josef-Schneider-Strasse 2, 97080 Würzburg, Germany, DE |
| Abstract: | We report an unusual case of paternity testing from residues of chorionic villi 5 weeks after pregnancy termination. The autopsy of a 32-year-old female homicide victim revealed the presence of intact chorionic villi at the former placenta implantation site. Fetal cells were selectively isolated by laser-induced microdissection of the remaining villi to avoid contamination with maternal DNA. Simultaneous amplification of 12 STR loci in 2 PCR reactions resulted in a combined probability of paternity of 99.94%. This case demonstrates that laser-assisted microdissection and multiplex STR typing provide tools for paternity testing performed on endometrial mucosa long after the product of conception was removed by therapeutic abortion. Received: 2 May 2000 / Accepted: 7 November 2000 |
| Keywords: | Paternity testing Laser microdissection STR typing Abortion |
| 本文献已被 PubMed SpringerLink 等数据库收录！ | |