体外细胞培养观察咖啡酸锗对U14瘤细胞增殖抑制作用的研究

目的：探讨咖啡酸锗对U14瘤细胞增殖的抑制作用。方法：通过体外培养U14瘤细胞,四甲基偶氮唑盐（MTT）比色法检测0.01～10μg/ml终浓度咖啡酸锗对U14瘤细胞生长的抑制程度;台盼兰拒染法计算24h和48h后的细胞数量及细胞死亡率。结果：MTT比色法显示,咖啡酸锗各浓度对U14瘤细胞均有增殖抑制作用,增殖抑制率以1μg/ml时最显著（45.58%）,且呈一定的剂量依赖性。台盼兰染色计数培养24h及48h各用药组肿瘤细胞死亡率均升高,与阴性对照组比较具有明显差异（P〈0.01）。结论：咖啡酸锗对U14瘤细胞的增殖有明显抑制作用。

Study on antitumor effect of caffeic acid Ge to U14 cell in vitro

Objective：To evaluate the antitumor effect of caffeic acid Ge on U14 cell. Methods：MTT assay was applied to study the antitumor activities of caffeic acid Ge（0.01～10 μg/ml） in U14 cell lines in vitro. Trypan blue exclusion method and cultured for 24 hours and 48 hours after calculation the cell population and cell mortality. Results：The MTT results suggested that caffeic acid Ge exhibited high antiproliferative activity in U14 cell lines,than the best on 1 g/ml（45.58%）,and with dose-dependent relation in vitro.Trypan blue exclusion method and cultured for 24 hours and 48 hours after calculation the cell population and cell mortality,is significant difference between the NS group and caffeic acid-germanium groups（P〈0.01）. Conclusion：Caffeic acid Ge has antiproliferative effect on U14 tumor cells in vitro.