特异识别人脑乙酰胆碱酯酶抗原表位的单克隆抗体1F9

目的 :为了进一步研究乙酰胆碱酯酶的功能和结构以及精确地确定抗体对位与抗原表位的关系 ,我们采用单克隆抗体对人脑AChE的肽库进行筛选 ,希望能找到一对一的表位对位关系。方法 :采用MGeyson的方法化学合成人脑乙酰胆碱酯酶的十肽肽库 ,十肽N端生物素化后用于ELISA检测。结果 :我们用鼠抗电鳐乙酰胆碱酯酶的单抗 1F9筛选人脑乙酰胆碱酯酶的十肽肽库 ,得到 1个抗原表位 111。结论 :抗原表位 111可以被兔抗人脑AChE的多抗和鼠抗电鳐乙酰胆碱酯酶的单抗 1F9识别 ,表明此表位序列在人脑AChE和电鳐AChE中是高度保守的。

A monoclonal antibody 1F9 against Torpedo Acetylcholinesterase recognizes specifically an antigenic epitope of the hmnan brain Acetylcholinesterase

Objective:In order to research the relationship between the function and the structure of human brain Acetylcholinesterase,use the monocolonal antibodies scanning antigenic decapeptides of human brain Acetylcholinesterase.Methods:Synthesis of overlapping decapeptides corresponding to the sequence of the human brain Acetylcholinesterase has been carried out on biotinylated with primary amino groups according to a method developed by M Geyson. Peptides Synthesized using the multipincombinatorial chemical synthesis technique have been used in an enzyme linked immunoabsorbent assay.Results:A antigenic epitope of human brain Acetylcholinesterase recognized by monoclonal antibody against Torpedo Acetylcholinesterase is received by decapeptide scanning.Conclusion:The antigenic epitope 111 could be recognized by the polycolonal antibody against human brain Acetylcholinesterase and monoclonal antibody against Torpedo Acetylcholinesterase,the results indicate that the epitope is highly conserved in human brain AChE and Torpedo AChE.