慢性低灌注状态对大鼠脑部能量代谢及学习记忆功能的影响

目的探讨慢性低灌注状态下脑部能量代谢改变对认知功能的影响和可能的影响机制.方法雄性SD大鼠60只,随机分为3组:缺血1月组25只,缺血3月组25只,对照组10只.缺血组大鼠行双侧颈总动脉结扎,在试验的不同阶段对各组大鼠行水迷宫试验和跳台试验,并按期取脑行ATP酶、乳酸脱氢酶(LDH)组织化学染色.结果各缺血组大鼠在行为学功能上较对照组有显著减退,缺血3月组更为明显.各缺血组大鼠海马CA1区ATP酶活性显著降低、乳酸脱氢酶活性升高.结论经双侧颈总动脉永久结扎后形成的脑部慢性低灌注状态并导致的神经元能量代谢水平降低和功能紊乱可能引起认知功能减退.

Chronic cerebral hypoperfusion caused by permanent bilateral common carotid artery ligation:effects on cerebral energy metabolism and cognitive function

Objective To evaluate the cerebral energy metabolism and cognitive function change under chronic forebrain hypoperfusion status caused by permanent bilateral common carotid artery ligation. Methods Sixty male Sprague-Dawley rats were divided into 3 groups (control group,ischemia 1 month group,ischemia 3 months group) and rats of all ischemia groups undertook bilateral common carotid artery permanent ligation. Histochemical examination includes ATPase and lactate dehydrogenase (LDH) examination was performed in the end of the experiment.Results Compared with control group, each ischemia group had deficit in behavior testing and ischemia 3 months group was more distinct. In the hippocampus CA1 area of ischemia group rats, ATPase activity was significantly decreased and lactate dehydrogenase activity was significantly increased.Conclusions After permanent ligation of bilateral carotid artery, the perfusion of forebrain decreased and a chronic hypoperfusion status formed.Under such a hypoperfusion status, the subsequent inhibitory energy metabolism may lead to cognitive deficit.