| Apelin/APJ信号系统对脑缺血后血管内皮细胞修复及机制的研究 |
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| 引用本文： | 杨燚,高雨筱,温雅,武鹏,路帆.Apelin/APJ信号系统对脑缺血后血管内皮细胞修复及机制的研究[J].脑与神经疾病杂志,2021,29(3):138-142. |
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| 作者姓名： | 杨燚 高雨筱 温雅 武鹏 路帆 |
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| 作者单位： | 050000石家庄,河北医科大学第二医院神经内科,河北省神经病学实验室,河北省血管稳态重点实验室;050000石家庄,河北医科大学第二医院神经内科,河北省神经病学实验室,河北省血管稳态重点实验室;050000石家庄,河北医科大学第二医院神经内科,河北省神经病学实验室,河北省血管稳态重点实验室;050000石家庄,河北医科大学第二医院神经内科,河北省神经病学实验室,河北省血管稳态重点实验室;050000石家庄,河北医科大学第二医院神经内科,河北省神经病学实验室,河北省血管稳态重点实验室 |
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| 基金项目： | 国家自然科学基金(81601152);河北省教育厅青年基金项目(ON2019136);河北省卫健委重点科技研究计划(20190053)。 |
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| 摘 要： | 目的 探讨Apelin/APJ信号系统对脑缺血损伤的血管内皮细胞的修复作用.方法 采用电凝烧灼法制备局灶性大脑皮质梗死模型,通过侧脑室注射腺病毒Ad-Apelin或对照病毒Ad-RFP.实验动物随机分为4组,分别为假手术(sham)组、脑梗死组(MCAO)、Ad-Apelin+脑梗死组(Ad-Apelin)组、Ad-R...
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| 关 键 词： | Apelin/APJ信号系统 脑卒中 血-脑脊液屏障 血管新生 |
Effect and mechanism of Apelin/APJ signaling system on vascular endothelial cell repair after cerebral ischemia |
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| Yang Yi,Gao Yuxiao,Wen Ya,Wu Peng,Lu Fan.Effect and mechanism of Apelin/APJ signaling system on vascular endothelial cell repair after cerebral ischemia[J].Journal of Brain and Nervous Diseases,2021,29(3):138-142. |
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| Authors: | Yang Yi Gao Yuxiao Wen Ya Wu Peng Lu Fan |
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| Institution: | (Department of Neurology,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China) |
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| Abstract: | Objective To investigate the repair effect of Apelin/APJ signaling system on vascular endothelial cells after cerebral ischemia.Methods Focal cerebral cortex infarction models were prepared by electrocoagulation and cauterization.Adenovirus Ad-Apelin or control virus Ad-RFP were injected into the lateral ventricle.The experimental animals were randomly divided into four groups,namely sham operation group(Sham),cerebral infarction group(MCAO),Ad-Apelin+cerebral infarction group(Ad-Apelin)and Ad-RFP+cerebral infarction group(Ad-RFP).mNSS neurological function score,Western blot,immunohistochemistry,qPCR were used to detect the effect of Apelin on neural function,blood-brain barrier,angiogenesis and Notch,VEGF,AngⅠ/Ⅱsignaling pathways.Results Compared with Ad-RFP group,mNSS score,brain water content,and evans blue overflow of Ad-Apelin group were decreased.Compared with Ad-RFP group,the expression of CD105,which is the specific marker of new vascular endothelial cells,was upregulated in Ad-Apelin group.Expression of DLL4,VEGF and VEGFRⅡ,Notchl,AngⅠ,AngⅡin vascular endothelial cells were also upregulated.Conclusion Apelin protects the blood brain barrier and promote endothelial cell repair and angiogenesis through the Notch,VEGF,AngⅠ/Ⅱsignaling pathways. |
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| Keywords: | Apelin/APJ signaling system Stroke Blood brain barrier Angiogenesis |
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