植入式微波消融活体猪肾实验研究

目的通过研究植入式微波对正常活体猪肾实质的消融作用,为微波消融肾脏肿瘤的临床应用提供实验依据。方法采用水循环内冷式硬质微波针,微波发射频率为2450 MHz,能量输出50～80 W/600 s,观察不同能量条件下微波对活体猪肾实质的消融作用。结果植入式微波在不同能量条件下消融灶的形态均为椭球形,由消融中心向外周依次分别为组织炭化区、消融凝固区、充血反应区和正常组织区。不同区域病理表现各异。实验中分别统计了不同能量状态下消融范围（最大长径、宽径和体积）和天线周围温度变化。结论植入式微波消融技术可在活体猪肾实质形成类圆形消融坏死区,消融范围可随能量改变而变化。

Experimental study of implanted microwave ablation in vivo porcine kidney

Objective To investigate the ablation effects of implanted microwave on vivo porcine kidney and provide experimental foundation for microwave ablation renal tumor. Methods The rigid implanted microwave antenna with internally water-cooled was directly implanted into porcine kidney parenchyma in vivo. Microwave frequency was 2450 MHz. The energy output was 50-80 W/600 s. Ablations were performed in differential irradiation powers and time. Results The shapes of ablation lesions were all elliptical. The central area was composed of charred and ablated region. Histological examination revealed complete coagulation necrosis in this area. Beyond this ablation areA, hemorrhaging was scattered, and renal tubules were dilated in congestive region. In the circumferential region, the renal parenchyma showed a morphologically normal finding. The ablation volume, maximum longitudinal, transverse diameters of ablation lesions and the temperature change around the microwave antenna were analyzed. Conclusion The rigid implanted microwave antenna can produce reproducible elliptical ablation lesion in shape. This research provides technical foundation for the clinical application of the microwave ablation renal tumor.