淫羊藿水煎提取液促进鸡胚背根神经节神经突起生长和DNA、RNA合成的实验研究

４８ 枚鸡胚背根神经节随机分成６ 组体外培养。其中的１ 组为对照组，不用药。另５ 组为实验组，在培养液中加入淫羊藿提取液，浓度分别为０．２５％ 、０．５％ 、１％ 、２％ 、４％ 。观察测量神经突起长度和密度。取２４ 对神经节做配对实验，在每对中任 １ 枚培养液中加入３％ 的淫羊藿提取液，另１ 枚不加。培养结束前各取１２ 对分别用３ Ｈ Ｔｄｒ 和３ Ｈ Ｕｄｒ 标记。培养结束，测量神经突起长度、密度后，取出标本作同位素放射性测定。结果提示：淫羊藿水提取液能明显促进神经突起的生长，促进神经节的 Ｄ Ｎ Ａ、 Ｒ Ｎ Ａ合成。生长３ 天，各实验组神经突起长度分别是对照组的１．０４７、１．１０２、１．１７１、１．１９８、１．２７２ 倍，密度分别是对照组的１．１４４、１．２０９、１．４０６、１．４１４、１．４７９ 倍；配对实验显示，用药组神经突起长度、密度分别是对照组的１．１７０ 和 １．７３０ 倍；３ Ｈ Ｔｄｒ、３ Ｈ Ｕｄｒ 的参入强度分别是其对照组的１．５８８ 和１．３５４ 倍，差别显著。

AN EXPERIMENTAL STUDY ON STIMULATIVE EFFECT OF EXTRACT EPIMEDIUM ON THE GROWTH OF NEURAL AXON AND THE SYNSTHESIS OF DNA AND RNA OF THE GANGLIONS OF CHICK EMBRYO DORSAL ROOT

Forty eight ganglions of chick embryo dorsal root were randomly divided into 6 groups and in vitro cultivated. Of the 6 groups, Group 1 was used as the control group not treated by drug and other 5 groups were used as the experimental groups, to whose culture solutions were added Extract Epimedium with following concentrations: 0.25 %, 0.5 %, 1 %, 2%, and 4 %, respectively. The lengths and densities of the neural axons were observed and measured. Other 24 pairs of ganglions were used for paired experiment. 3 % Extract Epimedium was added to the culture solution of any of each pair, and nothing was added to that of the other of the pair. Before the culture end, the first and second 12 pairs of them were labelled with 3 H Tdr and 3 H Udr, respectively. After the culture end and the measurement of the length and density of neural axons, the samples were taken out for isotopic radioactive measurement. The results showed that Extract Epimedium could significantly stimulate the growth of the neural axons and the synsthesis of DNA and RNA in the ganglions. After 3 day growth, the axon lengths of the experimental groups were 1.047, 1.102, 1.171, 1.198 and 1.272 times of the control, respectively; and the densities were 1.144, 1.209, 1.406, 1.414 and 1.479 of the control, respecively. The paired experiment showed that the length and density of neural axons of the drug group were 1.170 and 1.730 times of the control; and the intakes of 3 H Tdr and 3 H Udr were 1.588 and 1.354 times of the control with a significant difference.