| 反相高效液相色谱法测定盐酸奥洛他定片的含量 |
| |
| 引用本文： | 崔志红,周庆英,王颖,张健翔.反相高效液相色谱法测定盐酸奥洛他定片的含量[J].中国药业,2010,19(17):31-32. |
| |
| 作者姓名： | 崔志红 周庆英 王颖 张健翔 |
| |
| 作者单位： | 1. 鲁南制药集团股份有限公司,山东,临沂,276006
2. 山东省临沂市药品检验所,山东,临沂,276001 |
| |
| 摘 要： | 目的采用反相高效液相色谱法测定盐酸奥洛他定片的含量。方法色谱柱为Zorbax SB-C18柱（250mm×4.5mm,5μm）,流动相为0.02mol/L磷酸二氢钠溶液（含0.2%辛烷基磺酸钠,用浓磷酸调pH至3.0）-乙腈（65∶35）,流速1.5mL/min,检测波长210nm,柱温为室温,进样量10μL。结果盐酸奥洛他定质量浓度在0.05～0.35g/L范围内与峰面积呈良好的线性关系（r=0.9999）,平均回收率为99.56%,RSD=0.72%（n=6）。结论所用方法专属性好,操作简便、快速,结果准确可靠,重现性好,可作为盐酸奥洛他定片的含量测定方法。
|
| 关 键 词： | 盐酸奥洛他定 含量测定 反相高效液相色谱法 |
Determination of Olopatadine Hydrochloride Tablets by RP-HPLC |
| |
| Cui Zhihong,Zhou Qingying,Wang Ying,Zhang Jianxian.Determination of Olopatadine Hydrochloride Tablets by RP-HPLC[J].China Pharmaceuticals,2010,19(17):31-32. |
| |
| Authors: | Cui Zhihong Zhou Qingying Wang Ying Zhang Jianxian |
| |
| Institution: | 1.Lunan Pharmaceutical Co.,Ltd.,Linyi,Shandong,China 276006; 2.Linyi Institute for Drug Control,Linyi,Shandong,China 276001) |
| |
| Abstract: | Objective To establish an RP-HPLC method for the determination of Olopatadine Hydrochloride Tablets.Methods The Zorbax SB-C18 column （250 mm×4.5 mm,5 μm） was used as analytical column.The mobile phase consisted of 0.02 mol/L NaH2PO4 containing 0.2%sodium 1-octane sulfonate （adjusting pH to 3.0 with H3PO4）-acetonitrile （65 ∶35） with the flow rate of 1.5 mL/min,the detection wavelength was 210 nm and the column temperature was at room temperature.The injection volume was 10 μL.Results The linear range for the content of olopatadine hydrochloride was 0.05-0.35 g/L（r=0.999 9）,the average recovery rate was 99.56%,RSD=0.72%（n=6）. Conclusion The method is simple,rapid,accurate,which is suitable for the content determination of Olopatadine Hydrochloride Tablets. |
| |
| Keywords: | olopatadine hydrochloride content determination RP-HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
