| 人卵泡颗粒细胞的原代培养与鉴定 |
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| 引用本文： | 孙艳,杜生荣,张文昌.人卵泡颗粒细胞的原代培养与鉴定[J].福建医科大学学报,2011,45(2):118-121. |
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| 作者姓名： | 孙艳 杜生荣 张文昌 |
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| 作者单位： | 孙艳,杜生荣,SUN Yan,DU Shengrong(福建医科大学教学医院,福建省妇幼保健院辅助生殖技术研究室,福州,350001);张文昌,ZHANG Wenchang(福建医科大学公共卫生学院,福州,350004) |
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| 摘 要： | 目的 建立和完善人卵泡颗粒细胞的体外原代培养和鉴定方法.方法 分离行辅助生殖助孕技术治疗的符合研究纳入标准的不孕患者的卵泡颗粒细胞,接种于DMEM培养基进行原代培养;以H-E染色和卵泡刺激素受体(FSHR)免疫组织化学染色对所培养的卵泡颗粒细胞进行鉴定.结果 分离培养的人卵泡颗粒细胞存活率>90%;FSHR免疫组织化学...
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| 关 键 词： | 粒层细胞 卵泡 细胞 培养的 免疫组织化学 |
Primary Culture and Identification of Human Folliole Granulosa Cells |
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| SUN Yan,DU Shengrong,ZHANG Wenchang.Primary Culture and Identification of Human Folliole Granulosa Cells[J].Journal of Fujian Medical University,2011,45(2):118-121. |
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| Authors: | SUN Yan DU Shengrong ZHANG Wenchang |
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| Institution: | SUN Yan1,DU Shengrong1,ZHANG Wenchang21.Center for Reproductive Medicine,Fujian Maternity and Child Health Hospital of Fujian Medical University Teaching Hospital,Fuzhou 350001,China,2.Public Health School of Fujian Medical University,Fuzhou 350004 |
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| Abstract: | Objective To establish and improve the primary culture in vitro and identification methods of human folliole granulosa cells.Methods Primary granulosa cells were obtained from women who were meeting the inclusion criteria and undergoing ART.Folliole granulosa cells were incubated in the DMEM medium.H-E staining and FSHR immunohistochemistry methods were used to identify folliole granulosa cells.Results Over 90% of the cultured cells were folliole granulosa cells.Cell purity was above 95% by FSHR immunohisto... |
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| Keywords: | granulosa cell folliole culture in vitro cell identification |
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