| 黄芪甲苷促进脐带血干细胞向心肌细胞分化作用的研究 |
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| 引用本文： | 廖联明,郑友生,江敏,赵素萍.黄芪甲苷促进脐带血干细胞向心肌细胞分化作用的研究[J].福建医药杂志,2011,33(1):1-4. |
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| 作者姓名： | 廖联明 郑友生 江敏 赵素萍 |
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| 作者单位： | 福建中医药大学附属第二人民医院,福州,350003 |
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| 摘 要： | 目的探讨黄芪甲苷对脐带血间充质干细胞向心肌细胞分化的作用。方法采用贴壁法分离人脐带血间充质干细胞,用流式细胞术对细胞表型进行鉴定。通过免疫组化方法观察黄芪甲苷对间充质干细胞向心肌细胞分化的促进作用。结果流式细胞仪检测结果表明,分离的脐带血间充质干细胞表达CD29、CD44和CD105,不表达CD34和HLA-DR。黄芪甲苷（浓度200 mg/L）作用下,间充质干细胞表达特异性心肌细胞蛋白,包括结蛋白（desmin）、α横纹肌肌动蛋白（-αsarcomeric actin）和肌钙蛋白T（C-TnT）。结论黄芪甲苷对脐带血间充质干细胞分化的促进作用,分化的心肌细胞在心肌损伤性疾病的潜在的治疗价值值得进一步研究。
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| 关 键 词： | 黄芪甲苷 间充质干细胞 心肌细胞 |
Effects of Astragaloside on differentiation of cord blood-derived mesenchymal stem cells toward cardiomyocytes |
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| LIAO Lian-ming,ZHENG You-sheng,JIANG Min,ZHAO Su-ping.Effects of Astragaloside on differentiation of cord blood-derived mesenchymal stem cells toward cardiomyocytes[J].Fujian Medical Journal,2011,33(1):1-4. |
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| Authors: | LIAO Lian-ming ZHENG You-sheng JIANG Min ZHAO Su-ping |
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| Institution: | LIAO Lian-ming,ZHENG You-sheng,JIANG Min,ZHAO Su-ping.The Second people's hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China |
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| Abstract: | Objective To evaluate whether Astragaloside could promote the differentiation of cord blood-derived mesenchymal stem cells toward cardiomyocytes.Methods Mesenchymal stem cells were isolated from cord blood by repeated plating.The phenotypes of stem cells were assayed by FACS.Differentiation of mesenchymal stem cells was shown by immunochemistry after stem cells were cultured in the presence of Astragaloside.Results FACS showed that cord blood-derived mesenchymal stem cells expressed high levels of CD29,CD44... |
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| Keywords: | Astragaloside mesenchymal stem cell cardiomyocytes |
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