Distribution of Diego blood group alleles and genotypes in the Chinese Korean population and sequence-based identification |
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| Institution: | 1. Blood Center of Jilin Province, Changchun 130033, China;2. Changchun Central Hospital, Changchun 130051, China;3. Hebei Medical University, 2014 Clinical Medicine, Shijiazhuang 050017, China;1. Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, Greece;2. Department of Biology, Section of Cell Biology and Biophysics, School of Science, National and Kapodistrian University of Athens, Greece;1. SH/Scientific-Hemostasis, Scientific Director and Consultant in Hemostasis and Thrombosis Diagnostics, Franconville, France;2. Hyphen BioMed, Neuville sur Oise, France;3. International Consultancy in Strategic Safety Improvements of Blood-Derived Bioproducts and Suppliers Quality Audit / Inspection, London, UK;1. International Consultancy in Strategic Advice on Safety Improvements of Blood-Derived Bioproducts and Suppliers Quality Audit / Inspection, London, UK;2. Romagna Transplant Network, Hematology Unit, Ravenna-I, Italy;1. Emory University, Department of Pathology and Laboratory Medicine, Atlanta, GA, USA;2. Biconcavity Inc., Retired USPHS Officer at Centers for Disease Control and Prevention,1106 Spring Mill Dr. SW, Lilburn, GA 30047, USA;3. Case Western Reserve University, University Hospitals Cleveland Medical Center, Department of Pathology, 11100 Euclid Avenue, Cleveland, OH 44106, USA;1. Stem Cell Mobilization and Collection Unit, Italy;2. PICC Team, IEO IRCCS, Milano, Italy;1. Department of Biochemistry, Hebrew University Faculty of Medicine, Jerusalem, Israel;2. Department of Hematology, Hadassah-Hebrew University Hospital, Jerusalem, Israel;3. Blood Bank, Hadassah-Hebrew University Hospital, Jerusalem, Israel;4. Department of Pediatric Surgery, Hadassah- Hebrew University Hospital, Jerusalem, Israel |
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| Abstract: | ObjectiveTo investigate Diego blood group alleles in the Chinese Korean population. The Diego blood group system plays an important role in transfusion medicine, but the distribution of the blood group in many Chinese ethnic populations remains unclear.MethodsSequence Specific Primer Polymerase Chain Reaction (SSP-PCR) was used for Diego genotyping and sequence-based typing PCR (PCR-SBT) was used to verify single nucleotide polymorphisms in the coding region of SLC4A1 starting from exon 19. Nine hundred and ten samples from the Chinese Korean population were investigated.ResultsThe frequency of the DI*01 and DI*02 alleles in the Chinese Korean population was 0.0516 and 0.9484, respectively. The most predominant genotype was DI*02/DI*02, with a frequency of 90.22 % (821/910). The frequency of DI*01/DI*02 was 9.23 % (84/910) and that of DI*01 /DI*01 was 0.55 % (5/910). The genotype distributions of the Diego blood group conformed to the Hardy-Weinberg equilibrium (P > 0.05).ConclusionThe data obtained from this study will be helpful for the creation of a donor database to provide antigen-negative blood to patients with allo-antibodies. Genotyping can be used as a substitute for the serological technique when antisera are unavailable and is suitable for screening a large number of donors for rare-blood-group databases. |
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| Keywords: | Allele frequency Diego blood group Genotyping SSP-PCR PCR-SBT Chinese Korean |
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