Dynamic expression of hepatic GP73 mRNA and protein and circulating GP73 during hepatocytes malignant transformation |
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| Institution: | 1. Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, China;2. Departments of Medical Immunology & Medical Informatics, Medical College of Nantong University, Nantong 226001, China;3. Department of Oncology, Nantong Hospital of Traditional Chinese Medicine, Nantong 226001, China;4. Department of Oncology, Affiliated Hospital of Nantong University, Nantong 226001, China;1. Endoscopy Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy;2. Endoscopy Unit, ASST Fatebenefratelli Sacco, Milan, Italy;3. Chirurgia Generale 1, ASST Fatebenefratelli Sacco, Milan, Italy;4. Endoscopy Division, Ospedale San Leopoldo Mandic di Merate, ASST Lecco, Lecco, Italy;5. Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”, Università degli Studi di Milano, Milan, Italy;6. Department of Emergency Medicine, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy;1. Department of Ultrasonography, Tongde Hospital of Zhejiang Province, Hangzhou 320012, China;2. Department of Ultrasonography, Zhejiang Cancer Hospital, Hangzhou 320022, China;3. Department of Radiology, Tongde Hospital of Zhejiang Province, Hangzhou 320012, China;4. Department of Pathology, Tongde Hospital of Zhejiang Province, Hangzhou 320012, China;5. Department of Ultrasound Medicine, the First Affliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China;1. Paediatric Liver, GI, Nutrition Centre and MowatLabs, King''s College Hospital NHS Foundation Trust, Denmark Hill, London SE5 9RS, UK;2. Department of Paediatric Oncology, The Royal Marsden Hospital, Sutton, London SE5 9RS, UK;3. Institute of Liver studies, King''s College Hospital NHS Foundation Trust, Denmark Hill, London SE5 9RS, UK |
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| Abstract: | BackgroundHepatic Golgi protein-73 (GP73) expression is related to hepatocellular carcinoma (HCC) progression. The aim of this study was to investigate the dynamic expression of GP73 mRNA and protein during hepatocytes malignant transformation.MethodsHuman GP73 expressions in 88 HCC tissues and their self-control surrounding tissues were examined by immunohistochemistry, and survival time of HCC patients was evaluated by the Kaplan-Meier method. HCC model of Sprague-Dawley rats was made by diet containing 2-fluorenylacetamide. The rats were divided into the control, hepatocyte degeneration, precanceration, and HCC groups to observe GP73 protein and mRNA alterations during hepatocytes malignant transformation.ResultsThe GP73 expression was significantly higher in the cancerous tissues than that in the surrounding tissues, with shorter survival time, and the positive rates of GP73 protein in human HCC tissues were 53.3% at stage I, 84.0% at stage II, 84.6% at stage III, and 60.0% at stage IV, respectively. The positive rates of hepatic GP73 protein and mRNA in the rat models were none in the control group, 66.7% and 44.4% in the hepatocytes degeneration group, 88.9% and 77.8% in the hepatocytes precanceration group, and 100% in the HCC group, respectively. There was a positive correlation (r = 0.91, P<0.01) between hepatic GP73 and serum GP73 during rat hepatocytes malignant transformation.ConclusionsAbnormal GP73 expression may be a sensitive and valuable biomarker in hepatocarcinogensis. |
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