粉防己碱和蝙蝠葛碱减低抗三尖杉酯碱的人白血病HL60细胞对阿霉素的抗性(英文)

目的:研究粉防己碱(Tet)和蝙蝠葛碱(Dau)能否减低抗三尖杉酯碱(Har)的人早幼粒白血病HL60抗性株对阿霉素(Dox)的抗性。方法:细胞计数法和克隆形成法测定药物毒性,流式细胞光度术分析细胞周期变化,荧光法测定Dox含量。结果:无细胞毒性的Tet和Dau明显地增强Dox对HL60抗性细胞的生长抑制作用,使克隆形成率从Dox单药的60％分别降低到0.2％,9.2％,使阻断在G_2M期的抗性细胞增多,但Tet和Dau不增强Dox对敏感的HL60细胞的毒性。Tet使胞内Dox积聚增加。结论:Tet和Dau减低抗Har的HL60细胞对Dox的抗性,其机制是增加Dox在细胞内积聚。

Reduction of doxorubicin resistance by tetrandrine and dauricine in harringtonine-resistant human leukemia (HL60) cells

AIM : To study whether tetrandrine (Tet) and dauricine (Dau) can reduce doxorubicin (Dox) resistance in the harringtonine (Har) -resistant human leukemia cells. METHODS: The drug cytotoxities were determined by counting cell numbers and colony formation. Cell cycle phases were assayed by flow cytometry, Dox contents were quantified by Dox fluorescence. RESULTS: The non-cytotoxic concentrations of Tet and Dau potentiated the growth-inhibitory actions of Dox in the Har-resist-ant HL60 cells. The colony formation effiencies were reduced from 60 % by Dox to 0.2 % by Tet Dox and 9.2 % by Dau Dox. Retardation of the G2M phase cells was increased. But Tet and Dau did not potentiate Dox cytotoxities in the sensitive HL60 cells. Dox accumulation in the Har-resistant HL60 cells treated by Tet was increased. CONCLUSION: Dox resistance in the Har-resistant HL60 cells treated by Tet or Dau was reduced, due to the increase of Dox accumulation in the cells.