胃交感传入神经元的节段性(HRP 法的研究)

<正> 内脏的感觉是由植物性神经传入,早在Langley 及 Pickinson 时已有研究,并被神经学者所公认。Ranson 著《神经系统解剖学》中谈到,“1914年 Chase 和 Ranson、1918年Ranson 和 Billingsley 等认为内脏传入纤维起源于脑脊神经节细胞,它们的纤维经过相应的脑脊神经、植物性神经及神经节,直接到达内脏。这些纤维粗细不等,包含粗细有髓维和纤许多无髓纤维”。Carpenter 也认

THE SEGMENTATION OF THE SYMPATHETIC AFFERENT NEURONS OF THE STOMACH(A study with horseradish peroxidase method)

Twenty seven rabbits and fifteen eats were used.To each animal 80-100μl of 10%horseradish peroxidase(HRP,Sigma VT)solution was injected into the tunica serosa of the stomach(anterior wall)of each animal.After a survial time of five days,the animals were sacrificed and their hearts were perfused with 2% paraformaldehyde solubed in 0.1M phosphate acid buffer(pH7.2-7.3). The spinal ganglia were treated according to HRP method and incubated in 3. 3-diominobenzidine(DAB)H_2O_2 solution.The distribution of the HRP labeled cells were examined under microscope and the results were as folllows: We could find clearly labeled cells appearing in the spinal ganglia from cervical 4(C4)to lumbars,ofwhich ganglia THS-9 were the segments where the labeled cells were the most numerous,cervical ganglia 4-8(C4-8)next,and thelumbus labeled cells were themost minimum.Moreover,it was found that the labeled cells of different sizes contained large cells and small cells, but the labeled cells were mainly small cells,and the large cells were comparatively less. A new point of view has been put forward by the above experimental studies.It suggested a new idea,i.e,the segment of the sympathetic afferent fibers of the stomach is longer(from cervical 4 to lumbus).