不同稀释方法对酶联免疫吸附法检测丙型肝炎病毒抗体结果的影响

［ 目的］ 研究不同的稀释方法对酶联免疫吸附法检测丙型肝炎病毒抗体结果的影响．［ 方法］应用酶联免疫吸附技术，采取３ 种不同的稀释方法，对６０ 份不同标本进行丙型肝炎病毒抗体检验．［ 结果］ 第一种方法阳性３４ 例，弱阳性１ 例，阴性２５ 例，第二种方法阳性３３ 例，阴性２７ 例，第三种方法阳性３２ 例，弱阳性１ 例，２７ 例为阴性．［ 结论］ 第一种方法比预测结果阳性率高，可出现假阳性结果，采用第二种方法与预测结果相符合，为最佳稀释方法，第三种方法与预测结果基本相符．

Effects of different dilution methods of enzyme immunoabsorption for detecting antibody of hepatitis C virus

OBJECTIVE To study the effects of different dilution methods of enzyme immunoabsorption for detecting antibody of hepatitis C virus.METHOD Using the enzyme immunoabsorption and selecting 3 different dilution methods, antibodies of hepatitis C virus were detected in 60 samples.RESULT There were 34 cases positive, 1 cases weak positive and 25 cases negative in first method; 33 cases positive and 27 cases negative in second method; 32 cases positiveu,1 cases weak positive and 27 cases negative in third method.CONCLUSION The first method is higher positive rate than the forecasted result and may present pseudo positive result. The second method accords with the forecasted result, therefore it is the best dilution method. The third method accords basically with the forecasted result..