伊凡思蓝在测定血视网膜屏障 破坏中的作用

目的探讨能否应用伊凡思蓝(Evans blue, EB)作为示踪物定量诊断血视网膜屏障破坏(blood retinal barrier breakdown, BRBB)。方法 实验动物选用雄性Sprague-Dawley大鼠。首先应用EB测定由血管内皮生长因子(vascular endothelial growth factor, VEGF)引起的视网膜血管渗漏。然后用EB测 量1周糖尿病导致的BRBB。以甲酰胺提取视网膜中的EB,用分光光度计测量提取液中的EB浓度。最后根据视网膜重量标准化视网膜内的EB含量。结果VEGF 处理眼视网膜内的EB含量显著高于未处理眼视网膜内的EB含量(P<0.0001)。早期糖尿病大鼠视网膜内的EB含量亦显著高于正常对照组视网膜内的EB含量(P<0.05)。结论EB是一种可以定量测定BRBB的敏感示踪物。(中华眼底病杂志,2001,17:221-223)

The effect of Evans blue on determining the breakdown of blood-retina barrier

Objective To verify the effect of Evans blue dye on determining the retina blood vessel leakage. Methods Male Sprague Dawley rats were used in this study. The VEGF induced retinal blood vessel leakage was checked with Evans blue dye. Then the blood retina barrier breakdown of 1 week diabe tic animals was quantified with Evans blue. The dye was extracted from retina by formamide and the extraction was checked with spectrophotometer. Evans blue leakage was normalized against wet or dry retina weight. Results The retinal Evans blue content of eyes treated with VEGF was remarkably higher than that of the controls (n=17, P<0.0001). And the eyes of 1 week diabetic duration animals had more Evans blue dye than that of the normal controls (P<0.05). Conclusion Evans blue dye is a sensitive tracer in quantitatively diagnosing the blood retina barrier breakdown.