血管内皮生长因子缓释微粒的实验研究

目的　探讨乳化-分散法合成血管内皮生长因子(vascular endothelial growth factor,VEGF)缓释微粒及其体外降解的情况。　方法　采用乳化-分散法,将可降解高分子聚乳酸/乙醇酸共聚物(polyactic glycolate acid,PL GA)对VEGF进行包裹,制成可控制释放微粒。应用酶联免疫吸附法(enzyme- linked immuno- sorbent assay,EL ISA)分别于第1、2和3个月检测缓释微粒体外释放VEGF的含量。　结果　5次实验共制得10 0份含VEGF的微粒,平均直径为0 .2 0～0 .33μm。用EL ISA法在降解的第1、2和3个月测得释放VEGF的平均含量分别为6 2±11、89±14和12 7±19ng/ L。　结论　乳化-分散法能合成可降解的含VEGF缓释微粒。

EXPERIMENTAL STUDY ON POLYACTIC GLYCOLATE ACID MICROPARTICLES WITH RELEASING-SLOWLY VASCULAR ENDOTHELIAL GROWTH FACTOR

OBJECTIVE: To observe the degradation of the polyactic glycolate acid (PLGA) microparticles with releasing-slowly vascular endothelial growth factor (VEGF) synthesized by the method of emulsification-diffusion. METHODS: The method of emulsification-diffusion is to incorporate VEGF into microparticles composed of biodegradable PLGA. The controlled release of microparticles are acquired. The content of the VEGF released slowly from PLGA microparticles in vitro was detected with ELISA at different time. RESULTS: We synthesized 100 releasing-slowly VEGF PLGA microparticles with the size of 0.20-0.33 microm by 5 times. The contents were 62 +/- 11 ng/L, 89 +/- 14 ng/L, and 127 +/- 19 ng/L in the 1st, the 2nd and the 3rd months after degradation, respectively. CONCLUSION: The PLGA microparticles with releasing-slowly VEGF can be synthesized by the method of emulsification-diffusion.