E‐cadherin downregulation and Twist overexpression since early stages of oral carcinogenesis |
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| Authors: | Brunno Santos de Freitas Silva Fernanda Paula Yamamoto‐Silva Hélder Antônio Rebelo Pontes Décio dos Santos Pinto Júnior |
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| Affiliation: | 1. Departament of Oral Pathology, Dental School, University of S?o Paulo, , S?o Paulo, Brazil;2. Department of Oral Medicine, Dental School, Federal University of Goiás, , Goiania, Brazil;3. Department of Stomatology (Oral Pathology), Jo?o de Barros University Hospital, Federal University of Pará, , Belém, Brazil |
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| Abstract: | There is some evidence of Twist participation in oral carcinogenesis; however, little is known about its interaction with E‐cadherin in oral squamous cell carcinoma (OSCC) development. This experimental study included an immunohistochemical analysis of Twist and E‐cadherin proteins in paraffin‐embedded specimens of oral leukoplakia (OL), OSCC, and normal oral mucosa. In addition, it was also performed a Western blot and double‐immunofluorescence analysis of Twist and E‐cadherin expression in OSCC cell lines. Significant differences in Twist and E‐cadherin immunoexpression were observed between normal oral mucosa and OL, with an inverse relation since the earliest stages of oral dysplasia (r = ?0,512; P < 0.001). Western blot and double‐immunofluorescence analysis showed differences in Twist and E‐cadherin expression among human oral keratinocytes and OSCC cell lines suggesting that downregulation of E‐cadherin occurs in a dependent manner of Twist in OSCC. Our results showed a possible value of Twist and E‐cadherin in the prediction of risk of oral epithelium malignant transformation. |
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| Keywords: | E‐cadherin oral cancer oral dysplasia oral leukoplakia oral squamous cell carcinoma Twist |
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