人疱疹病毒6型对EB病毒复制影响的研究

目的 探讨人疱疹病毒６型（ＨＨＶ－６）对ＥＢ病毒（ＥＢＶ）复制的影响。方法 用ＨＨＶ－６感染Ｒａｊｉ细胞和ＥＢＶ转化的淋巴细胞（ＬＣＬ），并用抗ＨＨＶ－６单克隆抗体（ＭＡｂ）作免疫萤光分析（ＩＦＡ）确定ＨＨＶ－６已感染这二种细胞。用抗ＥＢＶ人血清作ＩＦＡ观察ＥＢＶ抗原表达。结果 ＨＨＶ－６感染Ｒａｊｉ细胞后可见细胞病变效应（ＣＰＥ）；而ＬＣＬ未见ＣＰＥ。这二种细胞株用抗ＨＨＶ－６的ＭＡｂ作ＩＦＡ均

Study on the effect of human herpesvirus 6 on replication of Epstein-Barr virus]

OBJECTIVE: To examine the effect of human herpesvirus 6(HHV-6) on the replication of Epstein-Barr virus (EBV) in cell lines. METHODS: Both EBV-infected Raji cells and EBV-transformed lymphoblastoid cell lines (LCL) were infected with HHV-6. Immunofluoresence assay (IFA) with monoclonal antibodies (MAb) against HHV-6 was applied to confirm the infection of HHV-6 in the two cell lines. Expression of EBV antigen was examined by IFA using human anti-EBV serum. RESULTS: Following HHV-6 infection, cytopathic effects (CPE) were observed in Raji cells but not in LCL. HHV-6 were detected in both cell lines by IFA with anti-HHV-6 MAb, but not in controls. EBV antigens were detected by IFA with human serum against EBV in both HHV-6 infected cell lines. CONCLUSION: Data in this study suggest that HHV-6 promotes the expression of EBV antigen and may contribute to the pathogenesis of nasopharyngeal carcinoma in cooperation with EBV.